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CyFlow CD45RA Pacific Orange

品番 CF345450
抗体名 Anti-Hu CD45RA PAC OR,MEM-56
包装単位 100 tests
濃度 -
容量 0.4 ml
関連製品
(アイソタイプコントロール)
-
反応性|交差吸着 Human レーザー Violet
抗原 CD45RA, Leu18 最大蛍光波長 551 nm
クローン MEM-56 最大励起波長 400 nm
ホスト Mouse 標識/Format Pacific Orange™
アイソタイプ IgG2b 研究分野 Immunophenotyping | Phosphorylation
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD45RA PAC OR,MEM-56

特異性

The mouse monoclonal antibody MEM-56 recognizes CD45RA antigen, a 205-220 kDa single chain type I glycoprotein, isoform of the leukocyte common antigen (LCA). CD45RA is expressed on most of B lymphocytes, resting and native T lymphocytes, medullar thymocytes and monocytes.

抗原情報

CD45RA is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on naive T cells and normal plasma cells.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Bazil V, Horejsi V, Baudys M, Kristofova H, Strominger JL, Kostka W, Hilgert I: Biochemical characterization of a soluble form of the 53‑kDa monocyte surface antigen. Eur·J·Immunol. 1986·Dec; 16(12):1583‑9. <·PMID:·3493149·>
• Bhunia AK, Arai T, Bulkley G, Chatterjee S: Lactosylceramide mediates tumor necrosis factor‑alpha‑induced intercellular adhesion molecule‑1 (ICAM‑1 expression and the adhesion of neutrophil in human umbilical vein endothelial cells. J·Biol·Chem. 1998·Dec·18; 273(51):34349‑57. <·PMID:·9852101·>
• Cortez-Gonzalez X, Sidney J, Adotevi O, Sette A, Millard F, Lemonnier F, Langlade-Demoyen P, Zanetti M: Immunogenic HLA‑B7‑restricted peptides of hTRT. Int·Immunol. 2006·Dec; 18(12):1707‑18. <·PMID:·17077179·>
• Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ: Combinations of CD45 isoforms are crucial for immune function and disease. J·Immunol. 2006·Mar·15; 176(6):3417‑25. <·PMID:·16517710·>
• Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2008·Feb; 13(2):197‑212. <·PMID:·18157742·>
• Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le(X) but is inhibited by sLe(X). Biochem·J. 2007·Sep·1; 406(2):333‑41. <·PMID:·17497959·>
• Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·>