CyFlow CD64 Alexa Fluor 647
| 品番 | CC859263 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD64 AF 647,10.1 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 0.4 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 Alexa Fluor™ 647 (AP602054) |
||
| 反応性|交差吸着 | Human | Non-Human Primates | レーザー | Red |
| 抗原 | CD64, FcγRI | 最大蛍光波長 | 665 nm |
| クローン | 10.1 | 最大励起波長 | 650 nm |
| ホスト | Mouse | 標識/Format | Alexa Fluor™ 647 |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD64 AF 647,10.1
特異性
The mouse monoclonal antibody 10.1 recognizes α subunit of CD64 antigen, a 72 kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes.
抗原情報
CD64 (FcγRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding α subunit and promiscuous γ subunit, which is indispensable for tyrosine-based signaling. However, even the α subunit can transduce signals leading to cellular effector functions. The isoform FcγRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcγRIb and FcγRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Beekman JM, Bakema JE, van der Linden J, Tops B, Hinten M, van Vugt M, van de Winkel JG, Leusen JH: Modulation of FcgammaRI (CD64) ligand binding by blocking peptides of periplakin. J·Biol·Chem. 2004·Aug·6; 279(32):33875‑81. <·PMID:·15161926·>
• Brichard B, Varis I, Latinne D, Deneys V, de Bruyere M, Leveugle P, Cornu G: Intracellular cytokine profile of cord and adult blood monocytes. Bone·Marrow·Transplant. 2001·May; 27(10):1081‑6. <·PMID:·11438825·>
• Devaraj S, Davis B, Simon SI, Jialal I: CRP promotes monocyte‑endothelial cell adhesion via Fcgamma receptors in human aortic endothelial cells under static and shear flow conditions. Am·J·Physiol·Heart·Circ·Physiol. 2006·Sep; 291(3):H1170‑6. <·PMID:·16603696·>
• Devaraj S, Du Clos TW, Jialal I: Binding and internalization of C‑reactive protein by Fcgamma receptors on human aortic endothelial cells mediates biological effects. Arterioscler·Thromb·Vasc·Biol. 2005·Jul; 25(7):1359‑63. <·PMID:·15860734·>
• Dougherty GJ, Selvendran Y, Murdoch S, Palmer DG, Hogg N: The human mononuclear phagocyte high‑affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1. Eur·J·Immunol. 1987·Oct; 17(10):1453‑9. <·PMID:·3500057·>
• Fadlon E, Vordermeier S, Pearson TC, Mire-Sluis AR, Dumonde DC, Phillips J, Fishlock K, Brown KA: Blood polymorphonuclear leukocytes from the majority of sickle cell patients in the crisis phase of the disease show enhanced adhesion to vascular endothelium and increased expression of CD64. Blood. 1998·Jan·1; 91(1):266‑74. <·PMID:·9414294·>
• Hashimoto S, Yamada M, Motoyoshi K, Akagawa KS: Enhancement of macrophage colony‑stimulating factor‑induced growth and differentiation of human monocytes by interleukin‑10. Blood. 1997·Jan·1; 89(1):315‑21. <·PMID:·8978307·>
• Jayaram Y, Buckle AM, Hogg N: The Fc receptor, FcRI, and other activation molecules on human mononuclear phagocytes after treatment with interferon‑gamma. Clin·Exp·Immunol. 1989·Mar; 75(3):414‑20. <·PMID:·2495203·>
• Roura-Mir C, Wang L, Cheng TY, Matsunaga I, Dascher CC, Peng SL, Fenton MJ, Kirschning C, Moody DB: Mycobacterium tuberculosis regulates CD1 antigen presentation pathways through TLR‑2. J·Immunol. 2005·Aug·1; 175(3):1758‑66. <·PMID:·16034117·>
• Sánchez-Torres C, García-Romo GS, Cornejo-Cortés MA, Rivas-Carvalho A, Sánchez-Schmitz G: CD16+ and CD16‑ human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells. Int·Immunol. 2001·Dec; 13(12):1571‑81. <·PMID:·11717198·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN