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CyFlow CD117 PE

品番 CC022192
抗体名 Anti-Hu CD117 PE,104D2
包装単位 100 tests
濃度 -
容量 2 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 PE (BV655324)
反応性|交差吸着 Human | Non-Human Primates | Cow レーザー Blue, Green, Yellow
抗原 CD117, c-kit, SCFR 最大蛍光波長 576 nm
クローン 104D2 最大励起波長 496 nm, 565 nm
ホスト Mouse 標識/Format PE
アイソタイプ IgG1 研究分野 Immunophenotyping | Phosphorylation
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD117 PE,104D2

特異性

The mouse monoclonal antibody 104D2 recognizes extracellular part of CD117 antigen, a 145 kDa receptor tyrosine kinase.

抗原情報

CD117 (c-Kit) is a 145 kDa receptor tyrosine kinase that regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. Mutations of CD117 can lead to growth and progression of tumors. After binding of its ligand, SCF (stem cell factor), CD117 is autophosphorylated on its intracellular domains and activated. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells and various cancer cells, e.g. acute myeloid leukemia cells.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Blair A, Sutherland HJ: Primitive acute myeloid leukemia cells with long‑term proliferative ability in vitro and in vivo lack surface expression of c‑kit (CD117). Exp·Hematol. 2000·Jun; 28(6):660‑71. <·PMID:·10880752·>
• Broudy VC, Lin NL, Bühring HJ, Komatsu N, Kavanagh TJ: Analysis of c‑kit receptor dimerization by fluorescence resonance energy transfer. Blood. 1998·Feb·1; 91(3):898‑906. <·PMID:·9446650·>
• Broudy VC, Lin NL, Liles WC, Corey SJ, O'Laughlin B, Mou S, Linnekin D: Signaling via Src family kinases is required for normal internalization of the receptor c‑Kit. Blood. 1999·Sep·15; 94(6):1979‑86. <·PMID:·10477727·>
• Nagano M, Yamashita T, Hamada H, Ohneda K, Kimura K, Nakagawa T, Shibuya M, Yoshikawa H, Ohneda O: Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood. 2007·Jul·1; 110(1):151‑60. <·PMID:·17379743·>
• Rappold I, Ziegler BL, Köhler I, Marchetto S, Rosnet O, Birnbaum D, Simmons PJ, Zannettino AC, Hill B, Neu S, Knapp W, Alitalo R, Alitalo K, Ullrich A, Kanz L, Bühring HJ: Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase. Blood. 1997·Jul·1; 90(1):111‑25. <·PMID:·9207445·>
• Stevenson KS, McGlynn L, Hodge M, McLinden H, George WD, Davies RW, Shiels PG: Isolation, Characterisation and Differentiation of Thy1.1 sorted Pancreatic Adult Progenitor Cell Populations. Stem·Cells·Dev. 2009·Dec; 18(10):1389‑98. <·PMID:·19326970·>
• Wihlidal P, Varga F, Pfeilstöcker M, Karlic H: Expression and functional significance of osteocalcin splicing in disease progression of hematological malignancies. Leuk·Res. 2006·Oct; 30(10):1241‑8. <·PMID:·16387359·>
• Yoshino N, Ami Y, Terao K, Tashiro F, Honda M: Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti‑human cross‑reactive antibodies. Exp·Anim. 2000·Apr; 49(2):97‑110. <·PMID:·10889948·>
• Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD117 PE (104D2) CC022192 TDS (US)