特異性

The Armenian hamster monoclonal antibody MR-1 recognizes mouse CD154 / CD40L antigen, a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.

抗原情報

CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Fischbein MP, Ardehali A, Yun J, Schoenberger S, Laks H, Irie Y, Dempsey P, Cheng G, Fishbein MC, Bonavida B: CD40 signaling replaces CD4+ lymphocytes and its blocking prevents chronic rejection of heart transplants. J·Immunol. 2000·Dec·15; 165(12):7316‑22. <·PMID:·11120867·>
• Howard LM, Miga AJ, Vanderlugt CL, Dal Canto MC, Laman JD, Noelle RJ, Miller SD: Mechanisms of immunotherapeutic intervention by anti‑CD40L (CD154) antibody in an animal model of multiple sclerosis. J·Clin·Invest. 1999·Jan; 103(2):281‑90. <·PMID:·9916140·>
• Hwang Y, Nahm MH, Briles DE, Thomas D, Purkerson JM: Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L. Infect·Immun. 2000·Feb; 68(2):511‑7. <·PMID:·10639411·>
• Lode HN, Xiang R, Pertl U, Förster E, Schoenberger SP, Gillies SD, Reisfeld RA: Melanoma immunotherapy by targeted IL‑2 depends on CD4(+) T‑cell help mediated by CD40/CD40L interaction. J·Clin·Invest. 2000·Jun; 105(11):1623‑30. <·PMID:·10841521·>
• Luzina IG, Atamas SP, Storrer CE, daSilva LC, Kelsoe G, Papadimitriou JC, Handwerger BS: Spontaneous formation of germinal centers in autoimmune mice. J·Leukoc·Biol. 2001·Oct; 70(4):578‑84. <·PMID:·11590194·>
• Murakawa T, Kerklo MM, Zamora MR, Wei Y, Gill RG, Henson PM, Grover FL, Nicolls MR: Simultaneous LFA‑1 and CD40 ligand antagonism prevents airway remodeling in orthotopic airway transplantation: implications for the role of respiratory epithelium as a modulator of fibrosis. J·Immunol. 2005·Apr·1; 174(7):3869‑79. <·PMID:·15778341·>
• Noelle RJ, Roy M, Shepherd DM, Stamenkovic I, Ledbetter JA, Aruffo A: A 39‑kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc·Natl·Acad·Sci·USA. 1992·Jul·15; 89(14):6550‑4. <·PMID:·1378631·>
• Salgado CG, Nakamura K, Sugaya M, Tada Y, Asahina A, Koyama Y, Irie S, Tamaki K: Functional CD40 ligand is expressed on epidermal Langerhans cells. J·Leukoc·Biol. 1999·Aug; 66(2):281‑5. <·PMID:·10449168·>
• Shin T, Kennedy G, Gorski K, Tsuchiya H, Koseki H, Azuma M, Yagita H, Chen L, Powell J, Pardoll D, Housseau F: Cooperative B7‑1/2 (CD80/CD86) and B7‑DC costimulation of CD4+ T cells independent of the PD‑1 receptor. J·Exp·Med. 2003·Jul·7; 198(1):42613. <·PMID:·12847135·>
• Wu Y, Zhou Q, Zheng P, Liu Y: CD28‑independent induction of T helper cells and immunoglobulin class switches requires costimulation by the heat‑stable antigen. J·Exp·Med. 1998·Apr·6; 187(7):1151‑6. <·PMID:·9529332·>