CyFlow CD62L FITC
品番 | CA847650 | ||
---|---|---|---|
抗体名 | Anti-Hu CD62L FITC,DREG56 | ||
包装単位 | 100 tests | ||
濃度 | - | ||
容量 | 2 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG1 FITC (CD051682) |
||
反応性|交差吸着 | Human | レーザー | Blue |
抗原 | CD62L, L-selectin | 最大蛍光波長 | 518 nm |
クローン | DREG56 | 最大励起波長 | 490 to 495 nm |
ホスト | Mouse | 標識/Format | FITC |
アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD62L FITC,DREG56
特異性
The mouse monoclonal antibody DREG56 recognizes CD62L antigen, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells.
抗原情報
CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Abraham WM, Ahmed A, Sabater JR, Lauredo IT, Botvinnikova Y, Bjercke RJ, Hu X, Revelle BM, Kogan TP, Scott IL, Dixon RA, Yeh ET, Beck PJ: Selectin blockade prevents antigen‑induced late bronchial responses and airway hyperresponsiveness in allergic sheep. Am·J·Respir·Crit·Care·Med. 1999·Apr; 159(4‑1):1205‑14. <·PMID:·10194167·>
• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Jutila MA, Kurk S, Jackiw L, Knibbs RN, Stoolman LM: L‑selectin serves as an E‑selectin ligand on cultured human T lymphoblasts. J·Immunol. 2002·Aug·15; 169(4):1768‑73. <·PMID:·12165498·>
• Killock DJ, Parsons M, Zarrouk M, Ameer-Beg SM, Ridley AJ, Haskard DO, Zvelebil M, Ivetic A: In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L‑selectin. J·Biol·Chem. 2009·Mar·27; 284(13):8833‑45. <·PMID:·19129194·>
• Kishimoto TK, Jutila MA, Butcher EC: Identification of a human peripheral lymph node homing receptor: a rapidly down‑regulated adhesion molecule. Proc·Natl·Acad·Sci·USA. 1990·Mar; 87(6):2244‑8. <·PMID:·2179952·>
• Kishimoto TK, Warnock RA, Jutila MA, Butcher EC, Lane C, Anderson DC, Smith CW: Antibodies against human neutrophil LECAM‑1 (LAM‑1/Leu‑8/DREG‑56 antigen) and endothelial cell ELAM‑1 inhibit a common CD18‑independent adhesion pathway in vitro. Blood. 1991·Aug·1; 78(3):805‑11. <·PMID:·1713515·>
• Tu W, Mao H, Zheng J, Liu Y, Chiu SS, Qin G, Chan PL, Lam KT, Guan J, Zhang L, Guan Y, Yuen KY, Peiris JS, Lau YL: Cytotoxic T lymphocytes established by seasonal human influenza cross‑react against 2009 pandemic H1N1 influenza virus. J·Virol. 2010·Jul; 84(13):6527‑35. <·PMID:·20410263·>
• Xu T, Chen L, Shang X, Cui L, Luo J, Chen C, Ba X, Zeng X: Critical role of Lck in L‑selectin signaling induced by sulfatides engagement. J·Leukoc·Biol. 2008·Oct; 84(4):1192‑201. <·PMID:·18653462·>
• Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN