CyFlow FoxP3 Alexa Fluor 647
| 品番 | CA372958 | ||
|---|---|---|---|
| 抗体名 | Anti-H/M FoxP3 AF 647,3G3 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 0.5 mg/ml | ||
| 容量 | 0.2 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 Alexa Fluor™ 647 (AP602054) |
||
| 反応性|交差吸着 | Human | Mouse | レーザー | Red |
| 抗原 | FoxP3 | 最大蛍光波長 | 665 nm |
| クローン | 3G3 | 最大励起波長 | 650 nm |
| ホスト | Mouse | 標識/Format | Alexa Fluor™ 647 |
| アイソタイプ | IgG1 | 研究分野 | Transcription |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-H/M FoxP3 AF 647,3G3
特異性
The mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3 antigen, a 47-55 kDa transcription factor, which is the master regulator in the development and function of regulatory T cells.
抗原情報
FoxP3 (Forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and function of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome.
利用方法
The reagent is designed for Flow Cytometry analysis. Suggested working usage is 6 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Barnes MJ, Powrie F: Regulatory T cells reinforce intestinal homeostasis. Immunity. 2009·Sep·18; 31(3):401‑11. <·PMID:·19766083·>
• Bettini M, Vignali DA: Regulatory T cells and inhibitory cytokines in autoimmunity. Curr·Opin·Immunol. 2009·Dec; 21(6):612‑8. <·PMID:·19854631·>
• Elkord E: Novel therapeutic strategies by regulatory T cells in allergy. Chem·Immunol·Allergy. 2008; 94:150‑7. <·PMID:·18802345·>
• Gavin MA, Torgerson TR, Houston E, DeRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY: Single‑cell analysis of normal and FOXP3‑mutant human T cells: FOXP3 expression without regulatory T cell development. Proc·Natl·Acad·Sci·USA. 2006·Apr·25; 103(17):6659‑64. <·PMID:·16617117·>
• Kuhn A, Beissert S, Krammer PH: CD4(+)CD25 (+) regulatory T cells in human lupus erythematosus. Arch·Dermatol·Res. 2009·Jan; 301(1):71‑81. <·PMID:·18985367·>
• Lal G, Bromberg JS: Epigenetic mechanisms of regulation of Foxp3 expression. Blood. 2009·Oct·29; 114(18):3727‑35. <·PMID:·19641188·>
• Law JP, Hirschkorn DF, Owen RE, Biswas HH, Norris PJ, Lanteri MC: The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells. Cytometry·A. 2009·Dec; 75(12):1040‑50. <·PMID:·19845018·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN