CyFlow CD71 APC-Cy7
| 品番 | BZ847039 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD71 APC-CY7,MEM-75 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 0.4 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 APC-Cy7 (AQ870020) |
||
| 反応性|交差吸着 | Human | レーザー | Red |
| 抗原 | CD71, T9, TFR, TRFR | 最大蛍光波長 | 780 nm |
| クローン | MEM-75 | 最大励起波長 | 650 nm, 750 nm |
| ホスト | Mouse | 標識/Format | APC-Cy7 |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping | Endocytosis |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD71 APC-CY7,MEM-75
特異性
The mouse monoclonal antibody MEM-75 recognizes CD71 antigen, a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-75 does not block binding of transferrin to the receptor.
抗原情報
CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
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• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Grabbe S, Varga G, Beissert S, Steinert M, Pendl G, Seeliger S, Bloch W, Peters T, Schwarz T, Sunderkötter C, Scharffetter-Kochanek K: Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells. J·Clin·Invest. 2002·Jan; 109(2):183‑92. <·PMID:·11805130·>
• Graham RM, Chua AC, Herbison CE, Olynyk JK, Trinder D: Liver iron transport. World·J·Gastroenterol. 2007·Sep·21; 13(35):4725‑36. <·PMID:·17729394·>
• Rouas-Freiss N, Moreau P, Ferrone S, Carosella ED: HLA‑G proteins in cancer: do they provide tumor cells with an escape mechanism?. Cancer·Res. 2005·Nov·15; 65(22):10139‑44. <·PMID:·16287995·>
• Takeichi M: Cadherin cell adhesion receptors as a morphogenetic regulator. Science. 1991·Mar·22; 251(5000):1451‑5. <·PMID:·2006419·>
• Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN