特異性

The mouse monoclonal antibody MEM-258 recognizes an epitope on SCR4 (the membrane-proximal SCR) domain of CD46 antigen, a 56-66 kDa dimeric transmembrane protein expressed on T and B lymphocytes, platelets, monocytes, granulocytes, endothelial cells, epithelial cells and fibroblast; it is negative on erythrocytes.

抗原情報

CD46 (MCP; membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Fleischli C, Sirena D, Lesage G, Havenga MJ, Cattaneo R, Greber UF, Hemmi S: Species B adenovirus serotypes 3, 7, 11 and 35 share similar binding sites on the membrane cofactor protein CD46 receptor. J·Gen·Virol. 2007·Nov; 88(11):2925‑34. <·PMID:·17947513·>
• Fleischli C, Verhaagh S, Havenga M, Sirena D, Schaffner W, Cattaneo R, Greber UF, Hemmi S: The distal short consensus repeats 1 and 2 of the membrane cofactor protein CD46 and their distance from the cell membrane determine productive entry of species B adenovirus serotype 35. J·Virol. 2005·Aug; 79(15):10013‑22. <·PMID:·16014961·>
• Fremeaux-Bacchi V, Moulton EA, Kavanagh D, Dragon-Durey MA, Blouin J, Caudy A, Arzouk N, Cleper R, Francois M, Guest G, Pourrat J, Seligman R, Fridman WH, Loirat C, Atkinson JP: Genetic and functional analyses of membrane cofactor protein (CD46) mutations in atypical hemolytic uremic syndrome. J·Am·Soc·Nephrol. 2006·Jul; 17(7):2017‑25. <·PMID:·16762990·>
• Gaggar A, Shayakhmetov DM, Lieber A: CD46 is a cellular receptor for group B adenoviruses. Nat·Med. 2003·Nov; 9(11):1408‑12. <·PMID:·14566335·>
• Hoffmann D, Bayer W, Heim A, Potthoff A, Nettelbeck DM, Wildner O: Evaluation of twenty‑one human adenovirus types and one infectivity‑enhanced adenovirus for the treatment of malignant melanoma. J·Invest·Dermatol. 2008·Apr; 128(4):988‑98. <·PMID:·17960177·>
• Kälin S, Amstutz B, Gastaldelli M, Wolfrum N, Boucke K, Havenga M, DiGennaro F, Liska N, Hemmi S, Greber UF: Macropinocytotic uptake and infection of human epithelial cells with species B2 adenovirus type 35. J·Virol. 2010·May; 84(10):5336‑50. <·PMID:·20237079·>
• Liszewski MK, Kemper C, Price JD, Atkinson JP: Emerging roles and new functions of CD46. Springer·Semin·Immunopathol. 2005·Nov; 27(3):345‑58. <·PMID:·16200405·>
• Loré K, Adams WC, Havenga MJ, Precopio ML, Holterman L, Goudsmit J, Koup RA: Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses. J·Immunol. 2007·Aug·1; 179(3):1721‑9. <·PMID:·17641038·>
• Marie JC, Astier AL, Rivailler P, Rabourdin-Combe C, Wild TF, Horvat B: Linking innate and acquired immunity: divergent role of CD46 cytoplasmic domains in T cell induced inflammation. Nat·Immunol. 2002·Jul; 3(7):659‑66. <·PMID:·12055630·>
• Okada N, Liszewski MK, Atkinson JP, Caparon M: Membrane cofactor protein (CD46 is a keratinocyte receptor for the M protein of the group A streptococcus. Proc·Natl·Acad·Sci·USA. 1995·Mar·28; 92(7):2489‑93. <·PMID:·7708671·>
• Oliaro J, Pasam A, Waterhouse NJ, Browne KA, Ludford-Menting MJ, Trapani JA, Russell SM: Ligation of the cell surface receptor, CD46, alters T cell polarity and response to antigen presentation. Proc·Natl·Acad·Sci·USA. 2006·Dec·5; 103(49):18685‑90. <·PMID:·17116876·>
• Rebetz J, Na M, Su C, Holmqvist B, Edqvist A, Nyberg C, Widegren B, Salford LG, Sjögren HO, Arnberg N, Qian Q, Fan X: Fiber mediated receptor masking in non‑infected bystander cells restricts adenovirus cell killing effect but promotes adenovirus host co‑existence. PLoS·One. 2009·Dec·29; 4(12):e8484. <·PMID:·20041185·>
• Wang H, Tuve S, Erdman DD, Lieber A: Receptor usage of a newly emergent adenovirus type 14. Virology. 2009·May·10; 387(2):436‑41. <·PMID:·19307010·>
• Weyand NJ, Lee SW, Higashi DL, Cawley D, Yoshihara P, So M: Monoclonal antibody detection of CD46 clustering beneath Neisseria gonorrhoeae microcolonies. Infect·Immun. 2006·Apr; 74(4):2428‑35. <·PMID:·16552073·>