CyFlow CD36 PE
| 品番 | BZ332383 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD36 PE,CB38 (NL07) | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgM PE (CW330883) |
||
| 反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
| 抗原 | CD36, GPIV, GP3B | 最大蛍光波長 | 576 nm |
| クローン | CB38 (NL07) | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgM | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD36 PE,CB38 (NL07)
特異性
The mouse monoclonal antibody CB38 (NL07) recognizes CD36 antigen, a 85-113 kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes.
抗原情報
CD36 (FAT; fatty acid translocase) is an 85-113 kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and it is rapidly induced on Follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Alessio M, Ghigo D, Garbarino G, Geuna M, Malavasi F: Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07. Cell·Immunol. 1991·Oct·15; 137(2):487‑500. <·PMID:·1716523·>
• Alessio M, Greco NJ, Primo L, Ghigo D, Bosia A, Tandon NN, Ockenhouse CF, Jamieson GA, Malavasi F: Platelet activation and inhibition of malarial cytoadherence by the anti‑CD36 IgM monoclonal antibody NL07. Blood. 1993·Dec·15; 82(12):3637‑47. <·PMID:·7505121·>
• Alessio M, Roggero S, Bussolino F, Saitta M, Malavasi F: Characterization of the murine monoclonal antibody NL07 specific for the human thrombospondin receptor (CD36 molecule). Curr·Stud·Hematol·Blood·Transfus. 1991; 58:182‑6. <·PMID:·1720081·>
• Fadok VA, Warner ML, Bratton DL, Henson PM: CD36 is required for phagocytosis of apoptotic cells by human macrophages that use either a phosphatidylserine receptor or the vitronectin receptor (alpha v beta 3). J·Immunol. 1998·Dec·1; 161(11):6250‑7. <·PMID:·9834113·>
• Hoffmann PR, deCathelineau AM, Ogden CA, Leverrier Y, Bratton DL, Daleke DL, Ridley AJ, Fadok VA, Henson PM: Phosphatidylserine (PS) induces PS receptor‑mediated macropinocytosis and promotes clearance of apoptotic cells. J·Cell·Biol. 2001·Nov·12; 155(4):649‑59. <·PMID:·11706053·>
• Kapinsky M, Torzewski M, Büchler C, Duong CQ, Rothe G, Schmitz G: Enzymatically degraded LDL preferentially binds to CD14(high) CD16(+) monocytes and induces foam cell formation mediated only in part by the class B scavenger‑receptor CD36. Arterioscler·Thromb·Vasc·Biol. 2001·Jun; 21(6):1004‑10. <·PMID:·11397711·>
• Matasić R, Dietz AB, Vuk-Pavlović S: Dexamethasone inhibits dendritic cell maturation by redirecting differentiation of a subset of cells. J·Leukoc·Biol. 1999·Dec; 66(6):909‑14. <·PMID:·10614771·>
• McKenna RW, Washington LT, Aquino DB, Picker LJ, Kroft SH: Immunophenotypic analysis of hematogones (B‑lymphocyte precursors) in 662 consecutive bone marrow specimens by 4‑color flow cytometry. Blood. 2001·Oct·15; 98(8):2498‑507. <·PMID:·11588048·>
• Moniuszko M, Kowal K, Rusak M, Pietruczuk M, Dabrowska M, Bodzenta-Lukaszyk A: Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants. Clin·Vaccine·Immunol. 2006·Jun; 13(6):704‑7. <·PMID:·16760331·>
• Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN