CyFlow CD29 FITC
品番 | BZ181097 | ||
---|---|---|---|
抗体名 | Anti-Hu CD29 FITC,MEM-101A | ||
包装単位 | 100 tests | ||
濃度 | - | ||
容量 | 2 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG1 FITC (CD051682) |
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反応性|交差吸着 | Human | Pig | Dog | レーザー | Blue |
抗原 | CD29, Integrin β1 | 最大蛍光波長 | 518 nm |
クローン | MEM-101A | 最大励起波長 | 490 to 495 nm |
ホスト | Mouse | 標識/Format | FITC |
アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD29 FITC,MEM-101A
特異性
The mouse monoclonal antibody MEM-101A recognizes CD29 antigen, a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin α subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells).
抗原情報
CD29 (β1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different α chains (α1-α6, CD49a-f) determining the binding properties of β1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most β1 integrins correlates with acquiring multidrug resistance of tumor cells during selection in presence of antitumor drug. In platelets, translocation of intracellular pool of β1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a β1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1β.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Lundberg S, Lindholm J, Lindbom L, Hellström PM, Werr J: Integrin alpha2beta1 regulates neutrophil recruitment and inflammatory activity in experimental colitis in mice. Inflamm·Bowel·Dis. 2006·Mar; 12(3):172‑7. <·PMID:·16534417·>
• Morozevich GE, Kozlova NI, Preobrazhenskaya ME, Ushakova NA, Eltsov IA, Shtil AA, Berman AE: The role of beta1 integrin subfamily in anchorage‑dependent apoptosis of breast carcinoma cells differing in multidrug resistance. Biochemistry·(Mosc). 2006·May; 71(5):489‑95. <·PMID:·16732726·>
• Peng Q, Lai D, Nguyen TT, Chan V, Matsuda T, Hirst SJ: Multiple beta 1 integrins mediate enhancement of human airway smooth muscle cytokine secretion by fibronectin and type I collagen. J·Immunol. 2005·Feb·15; 174(4):2258‑64. <·PMID:·15699160·>
• Plánka L, Necas A, Srnec R, Rauser P, Starý D, Jancár J, Amler E, Filová E, Hlucilová J, Kren L, Gál P: Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs. Physiol·Res. 2009; 58(6):885‑93. <·PMID:·19093735·>
• Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL‑R1/DR4 to the plasma membrane. Apoptosis. 2008·Mar; 13(3):423‑36. <·PMID:·18165900·>
• Wencel-Drake JD, Dieter MG, Lam SC: Immunolocalization of beta 1 integrins in platelets and platelet‑derived microvesicles. Blood. 1993·Aug·15; 82(4):1197‑203. <·PMID:·7688992·>
• Werr J, Johansson J, Eriksson EE, Hedqvist P, Ruoslahti E, Lindbom L: Integrin alpha(2)beta(1 (VLA‑2 is a principal receptor used by neutrophils for locomotion in extravascular tissue. Blood. 2000·Mar·1; 95(5):1804‑9. <·PMID:·10688841·>
• Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN