研究用フローサイトと理化学分析

世界のリサーチ分野のお客さまへ先端の検査装置のご案内です。

お問い合わせはこちら

sysmex

研究用フローサイトと理化学分析sysmex

CyFlow CD178 PE

品番 BZ157747
抗体名 Anti-Hu CD178 PE,NOK-1
包装単位 100 tests
濃度 -
容量 1 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 PE (BV655324)
反応性|交差吸着 Human レーザー Blue, Green, Yellow
抗原 CD178, FasL, CD95L, APO1L 最大蛍光波長 576 nm
クローン NOK-1 最大励起波長 496 nm, 565 nm
ホスト Mouse 標識/Format PE
アイソタイプ IgG1 研究分野 Immunophenotyping | Apoptosis
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD178 PE,NOK-1

特異性

The mouse monoclonal antibody NOK-1 recognizes CD178 antigen, a 40 kDa transmembrane glycoprotein expressed on neutrophils, monocytes, and activated T and NK cells.

抗原情報

CD178 (Fas-L; Fas ligand or CD95L), a member of TNF family transmembrane glycoproteins, is responsible for induction of apoptosis in cells containing its receptor Fas/CD95. The CD178-mediated apoptosis pathway has been implicated in peripheral tolerance, tissue pathology, and maintenance of the immune privileged sites. Defects in this interaction may be related to some cases of systemic lupus erythematosus (SLE). CD178 was also described as a co-stimulatory receptor for T-cell activation in mice in vivo.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Chopin D, Barei-Moniri R, Maillé P, Le Frére-Belda MA, Muscatelli-Groux B, Merendino N, Lecerf L, Stoppacciaro A, Velotti F: Human urinary bladder transitional cell carcinomas acquire the functional Fas ligand during tumor progression. Am·J·Pathol. 2003·Apr; 162(4):1139‑49. <·PMID:·12651606·>
• Ghadimi MP, Sanzenbacher R, Thiede B, Wenzel J, Jing Q, Plomann M, Borkhardt A, Kabelitz D, Janssen O: Identification of interaction partners of the cytosolic polyproline region of CD95 ligand (CD178). FEBS·Lett. 2002·May·22; 519(1‑3):50‑8. <·PMID:·12023017·>
• Ji J, Chen JJ, Braciale VL, Cloyd MW: Apoptosis induced in HIV‑1‑exposed, resting CD4+ T cells subsequent to signaling through homing receptors is Fas/Fas ligand‑mediated. J·Leukoc·Biol. 2007·Jan; 81(1):297‑305. <·PMID:·17056762·>
• Jodo S, Pidiyar VJ, Xiao S, Furusaki A, Sharma R, Koike T, Ju ST: Fas ligand (CD178) cytoplasmic tail is a positive regulator of Fas ligand‑mediated cytotoxicity. J·Immunol. 2005·Apr·15; 174(8):4470‑4. <·PMID:·15814666·>
• Kayagaki N, Kawasaki A, Ebata T, Ohmoto H, Ikeda S, Inoue S, Yoshino K, Okumura K, Yagita H: Metalloproteinase‑mediated release of human Fas ligand. J·Exp·Med. 1995·Dec·1; 182(6):1777‑83. <·PMID:·7500022·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD178 PE (NOK-1) BZ157747 TDS (US)