CyFlow CD178 PE
品番 | BZ157747 | ||
---|---|---|---|
抗体名 | Anti-Hu CD178 PE,NOK-1 | ||
包装単位 | 100 tests | ||
濃度 | - | ||
容量 | 1 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
||
反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
抗原 | CD178, FasL, CD95L, APO1L | 最大蛍光波長 | 576 nm |
クローン | NOK-1 | 最大励起波長 | 496 nm, 565 nm |
ホスト | Mouse | 標識/Format | PE |
アイソタイプ | IgG1 | 研究分野 | Immunophenotyping | Apoptosis |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD178 PE,NOK-1
特異性
The mouse monoclonal antibody NOK-1 recognizes CD178 antigen, a 40 kDa transmembrane glycoprotein expressed on neutrophils, monocytes, and activated T and NK cells.
抗原情報
CD178 (Fas-L; Fas ligand or CD95L), a member of TNF family transmembrane glycoproteins, is responsible for induction of apoptosis in cells containing its receptor Fas/CD95. The CD178-mediated apoptosis pathway has been implicated in peripheral tolerance, tissue pathology, and maintenance of the immune privileged sites. Defects in this interaction may be related to some cases of systemic lupus erythematosus (SLE). CD178 was also described as a co-stimulatory receptor for T-cell activation in mice in vivo.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Chopin D, Barei-Moniri R, Maillé P, Le Frére-Belda MA, Muscatelli-Groux B, Merendino N, Lecerf L, Stoppacciaro A, Velotti F: Human urinary bladder transitional cell carcinomas acquire the functional Fas ligand during tumor progression. Am·J·Pathol. 2003·Apr; 162(4):1139‑49. <·PMID:·12651606·>
• Ghadimi MP, Sanzenbacher R, Thiede B, Wenzel J, Jing Q, Plomann M, Borkhardt A, Kabelitz D, Janssen O: Identification of interaction partners of the cytosolic polyproline region of CD95 ligand (CD178). FEBS·Lett. 2002·May·22; 519(1‑3):50‑8. <·PMID:·12023017·>
• Ji J, Chen JJ, Braciale VL, Cloyd MW: Apoptosis induced in HIV‑1‑exposed, resting CD4+ T cells subsequent to signaling through homing receptors is Fas/Fas ligand‑mediated. J·Leukoc·Biol. 2007·Jan; 81(1):297‑305. <·PMID:·17056762·>
• Jodo S, Pidiyar VJ, Xiao S, Furusaki A, Sharma R, Koike T, Ju ST: Fas ligand (CD178) cytoplasmic tail is a positive regulator of Fas ligand‑mediated cytotoxicity. J·Immunol. 2005·Apr·15; 174(8):4470‑4. <·PMID:·15814666·>
• Kayagaki N, Kawasaki A, Ebata T, Ohmoto H, Ikeda S, Inoue S, Yoshino K, Okumura K, Yagita H: Metalloproteinase‑mediated release of human Fas ligand. J·Exp·Med. 1995·Dec·1; 182(6):1777‑83. <·PMID:·7500022·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN