CyFlow CD73 PE
品番 | BY500144 | ||
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抗体名 | Anti-Hu CD73 PE,AD2 | ||
包装単位 | 100 tests | ||
濃度 | - | ||
容量 | 1 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
||
反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
抗原 | CD73, 5-nucleotidase | 最大蛍光波長 | 576 nm |
クローン | AD2 | 最大励起波長 | 496 nm, 565 nm |
ホスト | Mouse | 標識/Format | PE |
アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD73 PE,AD2
特異性
The mouse monoclonal antibody AD2 recognizes CD73 antigen, a 70 kDa GPI-anchored 5´-nucleotidase expressed predominantly on T and B cell subsets, follicular dendritic cells and endothelial cells.
抗原情報
CD73 (ecto-5´-nucleotidase) is a 70 kDa glycoprotein anchored to the extracellular leaflet of the plasma membrane by GPI. This ecto-enzyme catalyzes dephosphorylation of AMP to adenosine. CD73 is expressed in various types of cells, such as epithelial, muscle, and endothelial cells, neutrophils, lymphocytes and fibroblasts. Inflammatory mediators support CD73 expression and its enzymatic activity, leading to the release of adenosine, which modulates inflammation through adenosine receptors. CD73 is expressed in a variety of lymphomas and leukemias, including ALL and CLL, whereas immunodeficient patients usually express low levels of this protein.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Borrione P, Peola S, Mariani S, Besostri B, Mallone R, Malavasi F, Pileri A, Massaia M: CD38 stimulation lowers the activation threshold and enhances the alloreactivity of cord blood T cells by activating the phosphatidylinositol 3‑kinase pathway and inducing CD73 expression. J·Immunol. 1999·May·15; 162(10):6238‑46. <·PMID:·10229870·>
• Hashikawa T, Takedachi M, Terakura M, Saho T, Yamada S, Thompson LF, Shimabukuro Y, Murakami S: Involvement of CD73 (ecto‑5'‑nucleotidase) in adenosine generation by human gingival fibroblasts. J·Dent·Res. 2003·Nov; 82(11):888‑92. <·PMID:·14578500·>
• Kögler G, Sensken S, Airey JA, Trapp T, Müschen M, Feldhahn N, Liedtke S, Sorg RV, Fischer J, Rosenbaum C, Greschat S, Knipper A, Bender J, Degistirici O, Gao J, Caplan AI, Colletti EJ, Almeida-Porada G, Müller HW, Zanjani E, Wernet P: A new human somatic stem cell from placental cordblood with intrinsic pluripotent differentiation potential. J·Exp·Med. 2004·Jul·19; 200(2):123‑35. <·PMID:·15263023·>
• Mokry J, Soukup T, Micuda S, Karbanova J, Visek B, Brcakova E, Suchanek J, Bouchal J, Vokurkova D, Ivancakova R: Telomere attrition occurs during ex vivo expansion of human dental pulp stem cells. J·Biomed·Biotechnol. 2010; 2010:673513. <·PMID:·20976265·>
• Nemoto E, Tada H, Shimauchi H: Disruption of CD40/CD40 ligand interaction with cleavage of CD40 on human gingival fibroblasts by human leukocyte elastase resulting in down‑regulation of chemokine production. J·Leukoc·Biol. 2002·Sep; 72(3):538‑45. <·PMID:·12223522·>
• Paquet-Fifield S, Schlüter H, Li A, Aitken T, Gangatirkar P, Blashki D, Koelmeyer R, Pouliot N, Palatsides M, Ellis S, Brouard N, Zannettino A, Saunders N, Thompson N, Li J, Kaur P: A role for pericytes as microenvironmental regulators of human skin tissue regeneration. J·Clin·Invest. 2009·Sep; 119(9):2795‑806. <·PMID:·19652362·>
• Rodriguez MW, Paquet AC, Yang YH, Erle DJ: Differential gene expression by integrin beta 7+ and beta 7‑ memory T helper cells. BMC·Immunol. 2004·Jul·5; 5:13. <·PMID:·15236665·>
• Semenov OV, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch AH, Malek A: Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation. Am·J·Obstet·Gynecol. 2010·Feb; 202(2):193. <·PMID:·20035913·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN