CyFlow CD9 Alexa Fluor 647
| 品番 | BY471582 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD9 AF 647,MEM-61 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 0.4 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 Alexa Fluor™ 647 (AP602054) |
||
| 反応性|交差吸着 | Human | レーザー | Red |
| 抗原 | CD9, MRP-1, P24 | 最大蛍光波長 | 665 nm |
| クローン | MEM-61 | 最大励起波長 | 650 nm |
| ホスト | Mouse | 標識/Format | Alexa Fluor™ 647 |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD9 AF 647,MEM-61
特異性
The mouse monoclonal antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
抗原情報
CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Israels SJ, McMillan-Ward EM: Platelet tetraspanin complexes and their association with lipid rafts. Thromb·Haemost. 2007·Nov; 98(5):1081‑7. <·PMID:·18000614·>
• Kim YJ, Yu JM, Joo HJ, Kim HK, Cho HH, Bae YC, Jung JS: Role of CD9 in proliferation and proangiogenic action of human adipose‑derived mesenchymal stem cells. Pflugers·Arch. 2007·Nov; 455(2):283‑96. <·PMID:·17668233·>
• Lafleur MA, Xu D, Hemler ME: Tetraspanin proteins regulate membrane type‑1 matrix metalloproteinase‑dependent pericellular proteolysis. Mol·Biol·Cell. 2009·Apr; 20(7):2030‑40. <·PMID:·19211836·>
• Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, Minami S, Kijima T, Yoshida M, Kumagai T, Osaki T, Kawase I: Absence of CD9 enhances adhesion‑dependent morphologic differentiation, survival, and matrix metalloproteinase‑2 production in small cell lung cancer cells. Cancer·Res. 2006·Oct·1; 66(19):9557‑65. <·PMID:·17018612·>
• Singh AB, Sugimoto K, Dhawan P, Harris RC: Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance. Am·J·Physiol·Cell·Physiol. 2007·Nov; 293(5):C1660‑8. <·PMID:·17855771·>
• Stöckl J, Majdic O, Fischer G, Maurer D, Knapp W: Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA‑A1‑restricted, hapten‑specific CTL. J·Immunol. 2001·Sep·1; 167(5):2724‑33. <·PMID:·11509616·>
• Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc·Natl·Acad·Sci·USA. 2007·Jan·2; 104(1):234‑9. <·PMID:·17190803·>
• Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN