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CyFlow CD19 PerCP-Cy5.5

品番 BX932898
抗体名 Anti-Hu CD19 PERCP-CY5.5,4G7
包装単位 100 tests
濃度 -
容量 0.4 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 PerCP-Cy5.5 (CZ886982)
反応性|交差吸着 Human レーザー Blue, Green
抗原 CD19, B4 最大蛍光波長 695 nm
クローン 4G7 最大励起波長 482 nm
ホスト Mouse 標識/Format PerCP-Cy5.5
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD19 PERCP-CY5.5,4G7

特異性

The mouse monoclonal antibody 4G7 recognizes CD19 antigen, a 95 kDa type I transmembrane glycoprotein of immunoglobulin superfamily, expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.

抗原情報

CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

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• Basu S, Lynne CM, Ruiz P, Aballa TC, Ferrell SM, Brackett NL: Cytofluorographic identification of activated T‑cell subpopulations in the semen of men with spinal cord injuries. J·Androl. 2002·Jul-Aug; 23(4):551‑6. <·PMID:·12065463·>
• Dubois B, Massacrier C, Caux C: Selective attraction of naive and memory B cells by dendritic cells. J·Leukoc·Biol. 2001·Oct; 70(4):633‑41. <·PMID:·11590201·>
• Köller M, Zwölfer B, Steiner G, Smolen JS, Scheinecker C: Phenotypic and functional deficiencies of monocyte‑derived dendritic cells in systemic lupus erythematosus (SLE) patients. Int·Immunol. 2004·Nov; 16(11):1595‑604. <·PMID:·15381672·>
• Martino V, Tonelli R, Montemurro L, Franzoni M, Marino F, Fazzina R, Pession A: Down‑regulation of MLL‑AF9, MLL and MYC expression is not obligatory for monocyte‑macrophage maturation in AML‑M5 cell lines carrying t(9: 11)(p22: q23). Oncol·Rep. 2006·Jan; 15(1):207‑11. <·PMID:·16328057·>
• Muench MO, Roncarolo MG, Namikawa R: Phenotypic and functional evidence for the expression of CD4 by hematopoietic stem cells isolated from human fetal liver. Blood. 1997·Feb·15; 89(4):1364‑75. <·PMID:·9028960·>
• Porcellini S, Vallanti G, Nozza S, Poli G, Lazzarin A, Tambussi G, Siccardi AG, Grassi F: Improved thymopoietic potential in aviremic HIV infected individuals treated with HAART by intermittent IL‑2 administration. AIDS. 2003·Jul·25; 17(11):1621‑30. <·PMID:·12853744·>
• Stockmeyer B, Dechant M, van Egmond M, Tutt AL, Sundarapandiyan K, Graziano RF, Repp R, Kalden JR, Gramatzki M, Glennie MJ, van de Winkel JG, Valerius T: Triggering Fc alpha‑receptor I (CD8{09} recruits neutrophils as effector cells for CD20‑directed antibody therapy. J·Immunol. 2000·Nov·15; 165(10):5954‑61. <·PMID:·11067958·>
• Treusch M, Vonthein R, Baur M, Günaydin I, Koch S, Stübiger N, Eckstein AK, Peter HH, Ness T, Zierhut M, Kötter I: Influence of human recombinant interferon‑alpha2a (rhIFN‑alpha2a) on altered lymphocyte subpopulations and monocytes in Behcet's disease. Rheumatology·(Oxford). 2004·Oct; 43(10):1275‑82. <·PMID:·15252211·>
• Reinherz EL, Haynes BF, Nadler LM, Bernstein ID: Leukocyte Typing II, Volume 1 Human T Lymphocytes. Springer·Verlag,·New·York. 1986; 1:1‑549. <·PMID:·Springer·8587-5·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD19 PerCP-Cy5.5 (4G7) BX932898 TDS (US)