CyFlow CD44 PE
| 品番 | BX574152 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD44 PE,MEM-263 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 2 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
||
| 反応性|交差吸着 | Human | Pig | Dog | レーザー | Blue, Green, Yellow |
| 抗原 | CD44, H-CAM, Pgp-1 | 最大蛍光波長 | 576 nm |
| クローン | MEM-263 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD44 PE,MEM-263
特異性
The mouse monoclonal antibody MEM-263 recognizes extracellular (N-terminal) domain of standard CD44, a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
抗原情報
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumor growth and metastasis, antiapoptosis and directional motility of cancer cells.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Hollingsworth JW, Li Z, Brass DM, Garantziotis S, Timberlake SH, Kim A, Hossain I, Savani RC, Schwartz DA: CD44 regulates macrophage recruitment to the lung in lipopolysaccharide‑induced airway disease. Am·J·Respir·Cell·Mol·Biol. 2007·Aug; 37(2):248‑53. <·PMID:·17446529·>
• Liu J, Bi G, Wen P, Yang W, Ren X, Tang T, Xie C, Dong W, Jiang G: Down‑regulation of CD44 contributes to the differentiation of HL‑60 cells induced by ATRA or HMBA. Cell·Mol·Immunol. 2007·Feb; 4(1):59‑63. <·PMID:·17349212·>
• Subramaniam V, Gardner H, Jothy S: Soluble CD44 secretion contributes to the acquisition of aggressive tumor phenotype in human colon cancer cells. Exp·Mol·Pathol. 2007·Dec; 83(3):341‑6. <·PMID:·17945213·>
• Subramaniam V, Vincent IR, Gardner H, Chan E, Dhamko H, Jothy S: CD44 regulates cell migration in human colon cancer cells via Lyn kinase and AKT phosphorylation. Exp·Mol·Pathol. 2007·Oct; 83(2):207‑15. <·PMID:·17599831·>
• Vigetti D, Viola M, Karousou E, Rizzi M, Moretto P, Genasetti A, Clerici M, Hascall VC, De Luca G, Passi A: Hyaluronan‑CD44‑ERK1/2 regulate human aortic smooth muscle cell motility during aging. J·Biol·Chem. 2007·Dec·12; <·PMID:·20811967·>
• McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN