特異性

The mouse monoclonal antibody MEM-154 reacts with an epitope on CD16 antigen that is residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcγRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes.

抗原情報

CD16 (FcγRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcγRIII is expressed in two forms - FcγRIIIA and FcγRIIIB. FcγRIIIA is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcεRI-γ subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcγRIIIA is associated, moreover, with FcεRI-β subunit. Besides IgG, FcγRIIIA can be triggered also by oligomeric IgE. FcγRIIIB is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

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• de Haas M, Koene HR, Kleijer M, de Vries E, Simsek S, van Tol MJ, Roos D, von dem Borne AE: A triallelic Fc gamma receptor type IIIA polymorphism influences the binding ofhuman IgG by NK cell Fc gamma RIIIa. J·Immunol. 1996·Apr·15; 156(8):2948‑55. <·PMID:·8609432·>
• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Gasdaska JR, Sherwood S, Regan JT, Dickey LF: An afucosylated anti‑CD20 monoclonal antibody with greater antibody‑dependent cellular cytotoxicity and B‑cell depletion and lower complement‑dependent cytotoxicity than rituximab. Mol·Immunol. 2012·Mar; 50(3):134‑41. <·PMID:·22305040·>
• Gessner JE, Grussenmeyer T, Kolanus W, Schmidt RE: The human low affinity immunoglobulin G Fc receptor III‑A and III‑B genes: Molecular characterization of the promoter regions. J·Biol·Chem. 1995·Jan·20; 270(3):1350‑61. <·PMID:·7836402·>
• Kocher M, Siegel ME, Edberg JC, Kimberly RP: Cross‑linking of Fc gamma receptor IIa and Fc gamma receptor IIIb induces different proadhesive phenotypes on human neutrophils. J·Immunol. 1997·Oct·15; 159(8):3940‑8. <·PMID:·9378982·>
• Koene HR, Kleijer M, Algra J, Roos D, von dem Borne AE, de Haas M: Fc gammaRIIIa‑158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa‑48L/R/H phenotype. Blood. 1997·Aug·1; 90(3):1109‑14. <·PMID:·9242542·>
• Tamm A, Schmidt RE: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies: Implications for ligand binding. J·Immunol. 1996·Aug·15; 157(4):1576‑81. <·PMID:·8759741·>