特異性

The mouse monoclonal antibody 5A6G7 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allow to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.

抗原情報

利用方法

The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry and for Western blotting, ELISA and Immunocytochemistry. Suggested working usage is 1 µg/ml (for Western blotting). Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, Moreau P, Rouas-Freiss N, Carosella ED: Modulation of HLA‑G Expression in Human Neural Cells after Neurotropic Viral Infections. J·Virol. 2005·Dec; 79(24):15226‑15237. <·PMID:·16306594·>
• Le Rond S, Gonzalez A, Gonzalez AS, Carosella ED, Rouas-Freiss N: Indoleamine 2,3 dioxygenase and human leucocyte antigen‑G inhibit the T‑cell alloproliferative response through two independent pathways. Immunology. 2005·Nov; 116(3):297‑307. <·PMID:·16236119·>
• Le Rond S, Le Maoult J, Creput C, Menier C, Deschamps M, Le Friec G, Amiot L, Durrbach A, Dausset J, Carosella ED, Rouas-Freiss N: Alloreactive CD4+ and CD8+ T cells express the immunotolerant HLA‑G molecule in mixed lymphocyte reactions: in vivo implications in transplanted patients. Eur·J·Immunol. 2004·Mar; 34(3):649‑60. <·PMID:·14991594·>
• Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA‑G or HLA‑E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum·Immunol. 2003·Mar; 64(3):315‑26. <·PMID:·12590976·>
• Poláková K, Železníková T, Russ G: HLA‑G5 in the blood of leukemia patients and healthy individuals. Leuk·Res. 2013·Feb; 37(2):139‑45. <·PMID:·23146557·>
• Rebmann V, Lemaoult J, Rouas-Freiss N, Carosella ED, Grosse-Wilde H: Report of the Wet Workshop for Quantification of Soluble HLA‑G in Essen, 2004. Hum·Immunol. 2005·Aug; 66(8):853‑63. <·PMID:·16216668·>