CyFlow CD25 APC
| 品番 | BV354815 | ||
|---|---|---|---|
| 抗体名 | Anti-Ms CD25 APC,PC61.5 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 0.5 mg/ml | ||
| 容量 | 0.2 ml | ||
| 関連製品 (アイソタイプコントロール) |
- | ||
| 反応性|交差吸着 | Mouse | レーザー | Red |
| 抗原 | CD25, TAC, p55, IL-2R | 最大蛍光波長 | 660 nm |
| クローン | PC61.5 | 最大励起波長 | 650 nm |
| ホスト | Rat | 標識/Format | APC |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping | MHC |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Ms CD25 APC,PC61.5
特異性
The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes mouse CD25 antigen, a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
抗原情報
CD25 (IL2Rα, Tac) is a ligand-binding α subunit of interleukin 2 receptor (IL2R). Together with β and γ subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
利用方法
The reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Aiba Y, Hirayama F, Ogawa M: Clonal proliferation and cytokine requirement of murine progenitors for natural killer cells. Blood. 1997·Jun·1; 89(11):4005‑12. <·PMID:·9166839·>
• Jones CM, Cose SC, Coles RM, Winterhalter AC, Brooks AG, Heath WR, Carbone FR: Herpes simplex virus type 1‑specific cytotoxic T‑lymphocyte arming occurs within lymph nodes draining the site of cutaneous infection. J·Virol. 2000·Mar; 74(5):2414‑9. <·PMID:·10666272·>
• Kish DD, Gorbachev AV, Fairchild RL: CD8+ T cells produce IL‑2, which is required for CD(4+)CD25+ T cell regulation of effector CD8+ T cell development for contact hypersensitivity responses. J·Leukoc·Biol. 2005·Sep; 78(3):725‑35. <·PMID:·16000396·>
• Kotner J, Tarleton R: Endogenous CD4(+) CD25(+) regulatory T cells have a limited role in the control of Trypanosoma cruzi infection in mice. Infect·Immun. 2007·Feb; 75(2):861‑9. <·PMID:·17101658·>
• Tenorio EP, Olguín JE, Fernández J, Vieyra P, Saavedra R: Reduction of Foxp3+ cells by depletion with the PC61 mAb induces mortality in resistant BALB/c mice infected with Toxoplasma gondii. J·Biomed·Biotechnol. 2010; 2010:786078. <·PMID:·20037737·>
• Van VQ, Darwiche J, Raymond M, Lesage S, Bouguermouh S, Rubio M, Sarfati M: Cutting edge: CD47 controls the in vivo proliferation and homeostasis of peripheral CD4+ CD25+ Foxp3+ regulatory T cells that express CD103. J·Immunol. 2008·Oct·15; 181(8):5204‑8. <·PMID:·18832672·>
• Wei HX, Chuang YH, Li B, Wei H, Sun R, Moritoki Y, Gershwin ME, Lian ZX, Tian Z: CD4+ CD25+ Foxp3+ regulatory T cells protect against T cell‑mediated fulminant hepatitis in a TGF‑beta‑dependent manner in mice. J·Immunol. 2008·Nov·15; 181(10):7221‑9. <·PMID:·18981144·>
• Yu CT, Feng MH, Shih HM, Lai MZ: Increased p300 expression inhibits glucocorticoid receptor‑T‑cell receptor antagonism but does not affect thymocyte positive selection. Mol·Cell·Biol. 2002·Jul; 22(13):4556‑66. <·PMID:·12052865·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN