CyFlow CD6 Pacific Blue
| 品番 | BV249149 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD6 PAC BLUE,MEM-98 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 0.4 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 Pacific Blue™ (AP887259) |
||
| 反応性|交差吸着 | Human | レーザー | Violet |
| 抗原 | CD6, T12 | 最大蛍光波長 | 455 nm |
| クローン | MEM-98 | 最大励起波長 | 400 to 410 nm |
| ホスト | Mouse | 標識/Format | Pacific Blue™ |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD6 PAC BLUE,MEM-98
特異性
The mouse monoclonal MEM-98 antibody recognizes CD6 antigen, a 100-130 kDa single chain transmembrane glycoprotein expressed on T and B lymphocytes subsets, thymocytes, and acute lymphocytic leukemia cells.
抗原情報
CD6 (T12) is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6 interacts with its ligand CD166 (ALCAM; activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. CD6 may exert some its functions via association with CD5, probably by fine-tuning CD5 signaling. Ligation of CD6 has antiapoptotic role in chronic lymphocytic leukemia B cells.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Bazil V, Horejsi V, Baudys M, Kristofova H, Strominger JL, Kostka W, Hilgert I: Biochemical characterization of a soluble form of the 53‑kDa monocyte surface antigen. Eur·J·Immunol. 1986·Dec; 16(12):1583‑9. <·PMID:·3493149·>
• Castro MA, Oliveira MI, Nunes RJ, Fabre S, Barbosa R, Peixoto A, Brown MH, Parnes JR, Bismuth G, Moreira A, Rocha B, Carmo AM: Extracellular isoforms of CD6 generated by alternative splicing regulate targeting of CD6 to the immunological synapse. J·Immunol. 2007·Apr·1; 178(7):4351‑61. <·PMID:·17371992·>
• Hassan NJ, Simmonds SJ, Clarkson NG, Hanrahan S, Puklavec MJ, Bomb M, Barclay AN, Brown MH: CD6 regulates T‑cell responses through activation‑dependent recruitment of the positive regulator SLP‑76. Mol·Cell·Biol. 2006·Sep; 26(17):6727‑38. <·PMID:·16914752·>
• Osorio LM, De Santiago A, Aguilar-Santelises M, Mellstedt H, Jondal M: CD6 ligation modulates the Bcl‑2/Bax ratio and protects chronic lymphocyticleukemia B cells from apoptosis induced by anti‑IgM. Blood. 1997·Apr·15; 89(8):2833‑41. <·PMID:·9108402·>
• Zimmerman AW, Joosten B, Torensma R, Parnes JR, van Leeuwen FN, Figdor CG: Long‑term engagement of CD6 and ALCAM is essential for T‑cell proliferationinduced by dendritic cells. Blood. 2006·Apr·15; 107(8):3212‑20. <·PMID:·16352806·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN