特異性

The mouse monoclonal antibody W6/32 recognises MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analyzing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. The W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.

抗原情報

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant β2-microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Barnstable CJ, Bodmer WF, Brown G, Galfre G, Milstein C, Williams AF, Ziegler A: Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens ‑ new tools for genetic analysis. Cell. 1978; 14:9‑20. <·PMID:·667938·>
• Brodsky FM, Parham P: Evolution of HLA antigenic determinants: species cross reactions of monoclonal antibodies. Immunogenetics. 1982; 15:151‑166. <·PMID:·6174442·>
• Jacobsen CN, Aasted B, Broe MK, Petersen JL: Reactivities of 20 anti‑human monclonal antibodies with leucocytes from ten different animal species. Vet·Immunopathol. 1993; 39:461‑466. <·PMID:·8116221·>
• Kievits F, Ivanyi P: Monomorphic anti‑HLA monoclonal antibody (W6/32) recognizes polymorphic H‑2 heavy‑chain determinants exposed by association with bovine or human but not murine beta 2‑microglobulin.. Hum·Immunol. 1987·Oct; 20(2):115‑26. <·PMID:·3316135·>
• Ladasky JJ, Shum BP, Canavez F, Seuanez HN, Parham P: Residue 3 of beta2‑microglobulin affects binding of class I MHC molecules by the W6/32 antibody. Immunogenetics. 1999·Apr; 49(4):312‑20. <·PMID:·10079295·>
• Le Discorde M, Moreau P, Sabatier P, Legeais JM, Carosella ED: Expression of HLA‑G in human cornea, an immune‑privileged tissue. Hum·Immunol. 2003·Nov; 64(11):1039‑44. <·PMID:·14602233·>
• Neefjes JJ, Hensen EJ, de Kroon TI, Ploegh HL: A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes. Immunogenetics. 1986; 23:341‑347. <·PMID:·3458670·>
• Shields MJ, Ribaudo RK: Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta‑microglobulin. Tissue·Antigens·. 1998·May; 51(5):567‑70. <·PMID:·9672158·>
• Stern PL, Beresford N, Friedman CI, Stevens VC, Risk JM, Johnson PM: Class I‑like MHC molecules expressed by baboon placental synctiotrophoblast. J·Immunol. 1987; 138(4):1088‑1091. <·PMID:·3543121·>
• Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V: The epitope recognized by pan‑HLA class I‑reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA‑B27/beta2‑microglobulin complex. Immunogenetics. 2001·Aug; 53(6):440‑6. <·PMID:·11685454·>