CyFlow CD8b FITC
| 品番 | BP937236 | ||
|---|---|---|---|
| 抗体名 | Anti-Rt CD8B FITC,341 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 0.5 mg/ml | ||
| 容量 | 0.2 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 FITC (CD051682) |
||
| 反応性|交差吸着 | Rat | レーザー | Blue |
| 抗原 | CD8b | 最大蛍光波長 | 518 nm |
| クローン | 341 | 最大励起波長 | 490 to 495 nm |
| ホスト | Mouse | 標識/Format | FITC |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping | MHC |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Rt CD8B FITC,341
特異性
The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b antigen, a 32-34 kDa β chain of the CD8 coreceptor, expressed on T cell subsets and some other cell types, such as macrophages.
抗原情報
The CD8b (CD8 β) subunit of CD8 T cell coreceptor is expressed in CD8 α/β heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 α/α homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 α/β but not α/α dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
利用方法
The reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Ellerman KE, Like AA: Islet cell membrane antigens activate diabetogenic CD4+ T‑cells in the BB/Wor rat. Diabetes. 1999·May; 48(5):975‑82. <·PMID:·10331400·>
• Hirji N, Lin TJ, Befus AD: A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J·Immunol. 1997·Feb·15; 158(4):1833‑40. <·PMID:·9029123·>
• Hirji N, Lin TJ, Bissonnette E, Belosevic M, Befus AD: Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major. J·Immunol. 1998·Jun·15; 160(12):6004‑11. <·PMID:·9637515·>
• Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur·J·Immunol. 1996·Nov; 26(11):2582‑6. <·PMID:·8921942·>
• Kühnlein P, Park JH, Herrmann T, Elbe A, Hünig T: Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. J·Immunol. 1994·Aug1; 153(3):979‑86. <·PMID:·8027567·>
• Lin TJ, Hirji N, Nohara O, Stenton GR, Gilchrist M, Befus AD: Mast cells express novel CD8 molecules that selectively modulate mediator secretion. J·Immunol. 1998·Dec·1; 161(11):6265‑72. <·PMID:·9834115·>
• Mabarrack NH, Turner NL, Mayrhofer G: Recent thymic origin, differentiation, and turnover of regulatory T cells. J·Leukoc·Biol. 2008·Nov; 84(5):1287‑97. <·PMID:·18682578·>
• Nohara O, Kulka M, Déry RE, Wills FL, Hirji NS, Gilchrist M, Befus AD: Regulation of CD8 expression in mast cells by exogenous or endogenous nitric oxide. J·Immunol. 2001·Nov·15; 167(10):5935‑9. <·PMID:·11698471·>
• Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hünig T: Differential thymus dependence of rat CD8 isoform expression. Eur·J·Immunol. 1992·Nov; 22(11):2841‑8. <·PMID:·1358623·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN