特異性

The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b antigen, a 32-34 kDa β chain of the CD8 coreceptor, expressed on T cell subsets and some other cell types, such as macrophages.

抗原情報

The CD8b (CD8 β) subunit of CD8 T cell coreceptor is expressed in CD8 α/β heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 α/α homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 α/β but not α/α dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Ellerman KE, Like AA: Islet cell membrane antigens activate diabetogenic CD4+ T‑cells in the BB/Wor rat. Diabetes. 1999·May; 48(5):975‑82. <·PMID:·10331400·>
• Hirji N, Lin TJ, Befus AD: A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J·Immunol. 1997·Feb·15; 158(4):1833‑40. <·PMID:·9029123·>
• Hirji N, Lin TJ, Bissonnette E, Belosevic M, Befus AD: Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major. J·Immunol. 1998·Jun·15; 160(12):6004‑11. <·PMID:·9637515·>
• Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur·J·Immunol. 1996·Nov; 26(11):2582‑6. <·PMID:·8921942·>
• Kühnlein P, Park JH, Herrmann T, Elbe A, Hünig T: Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. J·Immunol. 1994·Aug1; 153(3):979‑86. <·PMID:·8027567·>
• Lin TJ, Hirji N, Nohara O, Stenton GR, Gilchrist M, Befus AD: Mast cells express novel CD8 molecules that selectively modulate mediator secretion. J·Immunol. 1998·Dec·1; 161(11):6265‑72. <·PMID:·9834115·>
• Mabarrack NH, Turner NL, Mayrhofer G: Recent thymic origin, differentiation, and turnover of regulatory T cells. J·Leukoc·Biol. 2008·Nov; 84(5):1287‑97. <·PMID:·18682578·>
• Nohara O, Kulka M, Déry RE, Wills FL, Hirji NS, Gilchrist M, Befus AD: Regulation of CD8 expression in mast cells by exogenous or endogenous nitric oxide. J·Immunol. 2001·Nov·15; 167(10):5935‑9. <·PMID:·11698471·>
• Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hünig T: Differential thymus dependence of rat CD8 isoform expression. Eur·J·Immunol. 1992·Nov; 22(11):2841‑8. <·PMID:·1358623·>