CyFlow EGFR PE-Cy7
| 品番 | BM845157 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu EGFR PE-CY7,EGFR1 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 0.4 ml | ||
| 関連製品 (アイソタイプコントロール) |
- | ||
| 反応性|交差吸着 | Human | Horse | レーザー | Blue, Green, Yellow |
| 抗原 | EGFR | 最大蛍光波長 | 785 nm |
| クローン | EGFR1 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE-Cy7 |
| アイソタイプ | IgG2b | 研究分野 | Phosphorylation |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu EGFR PE-CY7,EGFR1
特異性
The mouse monoclonal antibody EGFR1 recognizes extracellular domain of human protein kinase EGFR; epitope within amino acids 6-273.
抗原情報
The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 or ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2/ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Aigner A, Juhl H, Malerczyk C, Tkybusch A, Benz CC, Czubayko F: Expression of a truncated 100 kDa HER2 splice variant acts as an endogenous inhibitor of tumour cell proliferation. Oncogene. 2001·Apr·19; 20(17):2101‑11. <·PMID:·11360194·>
• Akiyama M, Smith LT, Shimizu H: Changing patterns of localization of putative stem cells in developing human hair follicles. J·Invest·Dermatol. 2000·Feb; 114(2):321‑7. <·PMID:·10651993·>
• Fortunel NO, Hatzfeld JA, Rosemary PA, Ferraris C, Monier MN, Haydont V, Longuet J, Brethon B, Lim B, Castiel I, Schmidt R, Hatzfeld A: Long‑term expansion of human functional epidermal precursor cells: promotion of extensive amplification by low TGF‑beta1 concentrations. J·Cell·Sci. 2003·Oct·1; 116(19):4043‑52. <·PMID:·12953061·>
• Rebetz J, Tian D, Persson A, Widegren B, Salford LG, Englund E, Gisselsson D, Fan X: Glial progenitor‑like phenotype in low‑grade glioma and enhanced CD133‑expression and neuronal lineage differentiation potential in high‑grade glioma. PLoS·One. 2008·Apr·9; 3(4):e1936. <·PMID:·18398462·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN