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CyFlow CD44 PerCP-Cy5.5

品番 BM575398
抗体名 Anti-H/M CD44 PERCP-CY5.5,IM7
包装単位 0.1 mg
濃度 0.5 mg/ml
容量 0.2 ml
関連製品
(アイソタイプコントロール)
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反応性|交差吸着 Human | Mouse | Horse | Dog | Cat レーザー Blue, Green
抗原 CD44, H-CAM, Pgp-1 最大蛍光波長 695 nm
クローン IM7 最大励起波長 482 nm
ホスト Rat 標識/Format PerCP-Cy5.5
アイソタイプ IgG2b 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-H/M CD44 PERCP-CY5.5,IM7

特異性

The rat monoclonal antibody IM7 recognizes CD44 antigen, an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44.

抗原情報

CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumor growth and metastasis, antiapoptosis and directional motility of cancer cells.

利用方法

The reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Hegde VL, Singh NP, Nagarkatti PS, Nagarkatti M: CD44 mobilization in allogeneic dendritic cell‑T cell immunological synapse plays a key role in T cell activation. J·Leukoc·Biol. 2008·Jul; 84(1):134‑42. <·PMID:·18388297·>
• Katoh S, Matsumoto N, Kawakita K, Tominaga A, Kincade PW, Matsukura S: A role for CD44 in an antigen‑induced murine model of pulmonary eosinophilia. J·Clin·Invest. 2003·May; 111(10):1563‑70. <·PMID:·12750406·>
• Larkin J, Renukaradhya GJ, Sriram V, Du W, Gervay-Hague J, Brutkiewicz RR: CD44 differentially activates mouse NK T cells and conventional T cells. J·Immunol. 2006·Jul·1; 177(1):268‑79. <·PMID:·16785522·>
• Legg JW, Lewis CA, Parsons M, Ng T, Isacke CM: A novel PKC‑regulated mechanism controls CD44 ezrin association and directional cell motility. Nat·Cell·Biol. 2002·Jun; 4(6):399‑407. <·PMID:·12032545·>
• Lesley J, Trowbridge IS: Genetic characterization of a polymorphic murine cell‑surface glycoprotein. Immunogenetics. 1982·Mar; 15(3):313‑20. <·PMID:·7068175·>
• McKallip RJ, Do Y, Fisher MT, Robertson JL, Nagarkatti PS, Nagarkatti M: Role of CD44 in activation‑induced cell death: CD44‑deficient mice exhibit enhanced T cell response to conventional and superantigens. Int·Immunol. 2002·Sep; 14(9):1015‑26. <·PMID:·12202399·>
• Si-Tahar M, Sitaraman S, Shibahara T, Madara JL: Negative regulation of epithelium‑neutrophil interactions via activation of CD44. Am·J·Physiol·Cell·Physiol. 2001·Mar; 280(3):C423‑32. <·PMID:·11171560·>
• van Royen N, Voskuil M, Hoefer I, Jost M, de Graaf S, Hedwig F, Andert JP, Wormhoudt TA, Hua J, Hartmann S, Bode C, Buschmann I, Schaper W, van der Neut R, Piek JJ, Pals ST: CD44 regulates arteriogenesis in mice and is differentially expressed in patients with poor and good collateralization. Circulation. 2004·Apr·6; 109(13):1647‑5. <·PMID:·15023889·>
• Xu H, Manivannan A, Liversidge J, Sharp PF, Forrester JV, Crane IJ: Involvement of CD44 in leukocyte trafficking at the blood‑retinal barrier. J·Leukoc·Biol. 2002·Dec; 72(6):1133‑41. <·PMID:·12488494·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD44 PerCP-Cy5.5 (IM7) BM575398 TDS (US)