特異性

The mouse monoclonal antibody MEM-263 recognizes extracellular (N-terminal) domain of standard CD44, a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.

抗原情報

CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumor growth and metastasis, antiapoptosis and directional motility of cancer cells.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Hollingsworth JW, Li Z, Brass DM, Garantziotis S, Timberlake SH, Kim A, Hossain I, Savani RC, Schwartz DA: CD44 regulates macrophage recruitment to the lung in lipopolysaccharide‑induced airway disease. Am·J·Respir·Cell·Mol·Biol. 2007·Aug; 37(2):248‑53. <·PMID:·17446529·>
• Liu J, Bi G, Wen P, Yang W, Ren X, Tang T, Xie C, Dong W, Jiang G: Down‑regulation of CD44 contributes to the differentiation of HL‑60 cells induced by ATRA or HMBA. Cell·Mol·Immunol. 2007·Feb; 4(1):59‑63. <·PMID:·17349212·>
• Subramaniam V, Gardner H, Jothy S: Soluble CD44 secretion contributes to the acquisition of aggressive tumor phenotype in human colon cancer cells. Exp·Mol·Pathol. 2007·Dec; 83(3):341‑6. <·PMID:·17945213·>
• Subramaniam V, Vincent IR, Gardner H, Chan E, Dhamko H, Jothy S: CD44 regulates cell migration in human colon cancer cells via Lyn kinase and AKT phosphorylation. Exp·Mol·Pathol. 2007·Oct; 83(2):207‑15. <·PMID:·17599831·>
• Vigetti D, Viola M, Karousou E, Rizzi M, Moretto P, Genasetti A, Clerici M, Hascall VC, De Luca G, Passi A: Hyaluronan‑CD44‑ERK1/2 regulate human aortic smooth muscle cell motility during aging. J·Biol·Chem. 2007·Dec·12; <·PMID:·20811967·>
• McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·>