CyFlow CD16 FITC
品番 | BL185281 | ||
---|---|---|---|
抗体名 | Anti-Hu CD16 FITC,LNK16 | ||
包装単位 | 100 tests | ||
濃度 | - | ||
容量 | 2 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG1 FITC (CD051682) |
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反応性|交差吸着 | Human | Non-Human Primates | レーザー | Blue |
抗原 | CD16, FcγRIIIA, CD16a | 最大蛍光波長 | 518 nm |
クローン | LNK16 | 最大励起波長 | 490 to 495 nm |
ホスト | Mouse | 標識/Format | FITC |
アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD16 FITC,LNK16
特異性
The mouse monoclonal antibody LNK16 recognizes CD16 antigen, a low affinity receptor for aggregated IgG (FcγRIII antigen). CD16 exists in two different isoforms: CD16a (FcγRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcγRIIIB; 48 kDa; mainly expressed on neutrophils).
抗原情報
CD16 (FcγRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcγRIII is expressed in two forms - FcγRIIIA and FcγRIIIB. FcγRIIIA is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcεRI-γ subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcγRIIIA is associated, moreover, with FcεRI-β subunit. Besides IgG, FcγRIIIA can be triggered also by oligomeric IgE. FcγRIIIB is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Arase N, Arase H, Hirano S, Yokosuka T, Sakurai D, Saito T: IgE‑mediated activation of NK cells through Fc gamma RIII. J·Immunol. 2003·Mar·15; 170(6):3054‑8. <·PMID:·12626560·>
• Boyle JJ: Human macrophages kill human mesangial cells by Fas‑L‑induced apoptosis when triggered by antibody via CD16. Clin·Exp·Immunol. 2004·Sep; 137(3):529‑37. <·PMID:·15320902·>
• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Gessner JE, Grussenmeyer T, Kolanus W, Schmidt RE: The human low affinity immunoglobulin G Fc receptor III‑A and III‑B genes: Molecular characterization of the promoter regions. J·Biol·Chem. 1995·Jan·20; 270(3):1350‑61. <·PMID:·7836402·>
• Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL‑12p70 secretion and conveys strong T‑cell responses. BMC·Immunol. 2011·Jan·5; 12:2. <·PMID:·21208424·>
• Kocher M, Siegel ME, Edberg JC, Kimberly RP: Cross‑linking of Fc gamma receptor IIa and Fc gamma receptor IIIb induces different proadhesive phenotypes on human neutrophils. J·Immunol. 1997·Oct·15; 159(8):3940‑8. <·PMID:·9378982·>
• Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005·Nov·15; 106(10):3567‑74. <·PMID:·16046525·>
• Mathison RD, Befus AD, Davison JS, Woodman RC: Modulation of neutrophil function by the tripeptide feG. BMC·Immunol. 2003·Mar·4; 4:3. <·PMID:·12659660·>
• Tamm A, Schmidt RE: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies: Implications for ligand binding. J·Immunol. 1996·Aug·15; 157(4):1576‑81. <·PMID:·8759741·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN