CyFlow CD22 Pacific Blue
品番 | BK828088 | ||
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抗体名 | Anti-Hu CD22 PAC BLUE,IS7 | ||
包装単位 | 100 tests | ||
濃度 | - | ||
容量 | 0.4 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG1 Pacific Blue™ (AP887259) |
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反応性|交差吸着 | Human | レーザー | Violet |
抗原 | CD22, SIGLEC2, SIGLEC-2, BL-CAM | 最大蛍光波長 | 455 nm |
クローン | IS7 | 最大励起波長 | 400 to 410 nm |
ホスト | Mouse | 標識/Format | Pacific Blue™ |
アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD22 PAC BLUE,IS7
特異性
The mouse monoclonal antibody IS7 recognizes CD22 antigen, a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.
抗原情報
CD22 (Siglec-2; sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding α 2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Tateno H, Li H, Schur MJ, Bovin N, Crocker PR, Wakarchuk WW, Paulson JC: Distinct endocytic mechanisms of CD22 (Siglec‑2 and Siglec‑F reflect roles in cell signaling and innate immunity. Mol·Cell·Biol. 2007·Aug; 27(16):5699‑710. <·PMID:·17562860·>
• Tedder TF, Poe JC, Haas KM: CD22: A Multifunctional Receptor That Regulates B Lymphocyte Survival and Signal Transduction. Adv·Immunol. 2005; 88:1‑50. <·PMID:·16227086·>
• Walker JA, Smith KG: CD22: An inhibitory enigma. Immunology. 2008·Mar; 123(3):314‑25. <·PMID:·18067554·>
• Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN