CyFlow MHCII FITC
| 品番 | BJ649436 | ||
|---|---|---|---|
| 抗体名 | Anti-Ms MHCII FITC,M5/114 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 0.5 mg/ml | ||
| 容量 | 0.2 ml | ||
| 関連製品 (アイソタイプコントロール) |
- | ||
| 反応性|交差吸着 | Mouse | レーザー | Blue |
| 抗原 | MHC Class II | 最大蛍光波長 | 518 nm |
| クローン | M5/114 | 最大励起波長 | 490 to 495 nm |
| ホスト | Rat | 標識/Format | FITC |
| アイソタイプ | IgG2b | 研究分野 | Immunophenotyping | MHC |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Ms MHCII FITC,M5/114
特異性
The rat monoclonal antibody M5/114 recognizes mouse MHC class II glycoproteins (MHCII). It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
抗原情報
MHC (major histocompatibility complex) class II (MHCII) molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding groove between MHC class II α and β chain is open at both ends and is 15-24 amino acid residues long.
利用方法
The reagent is designed for Flow Cytometry analysis. Suggested working usage is 4 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Beers C, Burich A, Kleijmeer MJ, Griffith JM, Wong P, Rudensky AY: Cathepsin S controls MHC class II‑mediated antigen presentation by epithelial cells in vivo. J·Immunol. 2005·Feb·1; 174(3):1205‑12. <·PMID:·15661874·>
• Bhattacharya A, Dorf ME, Springer TA: A shared alloantigenic determinant on Ia antigens encoded by the I‑A and I‑E subregions: evidence for I region gene duplication. J·Immunol. 1981·Dec; 127(6):2488‑95. <·PMID:·6170707·>
• Clausen BE, Waldburger JM, Schwenk F, Barras E, Mach B, Rajewsky K, Förster I, Reith W: Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5‑deficient mice. Immunity. 1998·Feb; 8(2):143‑55. <·PMID:·9491996·>
• De Souza Leao S, Lang T, Prina E, Hellio R, Antoine JC: Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells. J·Cell·Sci. 1995·Oct; 108 (10):3219‑31. <·PMID:·7593283·>
• Kleijmeer M, Ramm G, Schuurhuis D, Griffith J, Rescigno M, Ricciardi-Castagnoli P, Rudensky AY, Ossendorp F, Melief CJ, Stoorvogel W, Geuze HJ: Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells. J·Cell·Biol. 2001·Oct·1; 155(1):53‑63. <·PMID:·11581285·>
• Kuwano Y, Prazma CM, Yazawa N, Watanabe R, Ishiura N, Kumanogoh A, Okochi H, Tamaki K, Fujimoto M, Tedder TF: CD83 influences cell‑surface MHC class II expression on B cells and other antigen‑presenting cells. Int·Immunol. 2007·Aug; 19(8):977‑92. <·PMID:·17804692·>
• Viville S, Neefjes J, Lotteau V, Dierich A, Lemeur M, Ploegh H, Benoist C, Mathis D: Mice lacking the MHC class II‑associated invariant chain. Cell. 1993·Feb·26; 72(4):635‑48. <·PMID:·7679955·>
• Zang W, Kalache S, Lin M, Schroppel B, Murphy B: MHC Class II‑mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C. J·Am·Soc·Nephrol. 2005·Dec; 16(12):3661‑8. <·PMID:·16221866·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN