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CyFlow SIT PE

品番 BJ327694
抗体名 Anti-Hu SIT PE,SIT-01
包装単位 0.1 mg
濃度 0.1 mg/ml
容量 1 ml
関連製品
(アイソタイプコントロール)
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反応性|交差吸着 Human レーザー Blue, Green, Yellow
抗原 SIT 最大蛍光波長 576 nm
クローン SIT-01 最大励起波長 496 nm, 565 nm
ホスト Mouse 標識/Format PE
アイソタイプ IgG1 研究分野 Adaptor proteins
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu SIT PE,SIT-01

特異性

The mouse monoclonal antibody SIT-01 recognizes SIT (SHP2-interacting transmembrane adaptor protein) expressed exclusively in lymphoid organs. It weakly crossreacts with mouse SIT.

抗原情報

SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.

利用方法

The reagent is designed for Flow Cytometry analysis. Suggested working usage is 2 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat·Rev·Immunol. 2004·Aug; 4(8):603‑16. <·PMID:·15286727·>
• Hubener C, Mincheva A, Lichter P, Schraven B, Bruyns E: Complete sequence, genomic organization, and chromosomal localization of the human gene encoding the SHP2‑interacting transmembrane adaptor protein (SIT). Immunogenetics. 2001·May-Jun; 53(4):337‑41. <·PMID:·11491537·>
• Marie-Cardine A, Kirchgessner H, Bruyns E, Shevchenko A, Mann M, Autschbach F, Ratnofsky S, Meuer S, Schraven B: SHP2‑interacting transmembrane adaptor protein (SIT), a novel disulfide‑linked dimer regulating human T cell activation. J·Exp·Med. 1999·Apr·19; 189(8):1181‑94. <·PMID:·10209036·>
• Simeoni L, Posevitz V, Kolsch U, Meinert I, Bruyns E, Pfeffer K, Reinhold D, Schraven B: The transmembrane adapter protein SIT regulates thymic development and peripheral T‑cell functions. Mol·Cell·Biol. 2005·Sep; 25(17):7557‑68. <·PMID:·16107703·>
• Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rudiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsi V: Transmembrane adaptor molecules: a new category of lymphoid‑cell markers. Blood. 2006·Jan·1; 107(1):213‑21. <·PMID:·16160011·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ SIT PE (SIT-01) BJ327694 TDS (US)