特異性

The mouse monoclonal antibody LCK-01 recognizes defined epitope (aa 22-36) of Lck antigen, a 56 kDa Src-family protein tyrosine kinase.

抗原情報

Lck is a Src-family tyrosine kinase, which is essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in its ζ subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by Lck and thereby activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to the plasma membrane, there is also a significant fraction associated with the Golgi apparatus, which may contribute to Raf activation under conditions of weak stimulation through the TCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

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• Cebecauer M, Cerny J, Horejsi V: Incorporation of leucocyte GPI‑anchored proteins and protein tyrosine kinases into lipid‑rich membrane domains of COS‑7 cells. Biochem·Biophys·Res·Commun. 1998·Feb·24; 243(3):706‑10. <·PMID:·9500981·>
• Denny MF, Kaufman HC, Chan AC, Straus DB: The lck SH3 domain is required for activation of the mitogen‑activated protein kinase pathway but not the initiation of T‑cell antigen receptor signaling. J·Biol·Chem. 1999·Feb·19; 274(8):5146‑52. <·PMID:·9988764·>
• Gruber C, Henkel M, Budach W, Belka C, Jendrossek V: Involvement of tyrosine kinase p56/Lck in apoptosis induction by anticancer drugs. Biochem·Pharmacol. 2004·May·15; 67(10):1859‑72. <·PMID:·15130763·>
• Hur YG, Yun Y, Won J: Rosmarinic acid induces p56lck‑dependent apoptosis in Jurkat and peripheral T cells via mitochondrial pathway independent from Fas/Fas ligand interaction. J·Immunol. 2004·Jan·1; 172(1):79‑87. <·PMID:·14688312·>
• Kim MJ, Park MT, Yoon CH, Byun JY, Lee SJ: Activation of Lck is critically required for sphingosine‑induced conformational activation of Bak and mitochondrial cell death. Biochem·Biophys·Res·Commun. 2008·May·30; 370(2):353‑8. <·PMID:·18371298·>
• Li M, Ong SS, Rajwa B, Thieu VT, Geahlen RL, Harrison ML: The SH3 domain of Lck modulates T‑cell receptor‑dependent activation of extracellular signal‑regulated kinase through activation of Raf‑1. Mol·Cell·Biol. 2008·Jan; 28(2):630‑41. <·PMID:·17998336·>
• Lovatt M, Filby A, Parravicini V, Werlen G, Palmer E, Zamoyska R: Lck regulates the threshold of activation in primary T cells, while both Lck and Fyn contribute to the magnitude of the extracellular signal‑related kinase response. Mol·Cell·Biol. 2006·Nov; 26(22):8655‑65. <·PMID:·16966372·>
• Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self‑MHC‑mediated Src family kinase activation. J·Immunol. 2007·Feb·15; 178(4):2262‑71. <·PMID:·17277131·>
• Romagnoli P, Strahan D, Pelosi M, Cantagrel A, van Meerwijk JP: A potential role for protein tyrosine kinase p56(lck) in rheumatoid arthritis synovial fluid T lymphocyte hyporesponsiveness. Int·Immunol. 2001·Mar; 13(3):305‑12. <·PMID:·11222499·>