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CyFlow CD11b APC-Cy7

品番 BE330247
抗体名 Anti-Hu CD11B APC-CY7,ICRF44
包装単位 100 tests
濃度 -
容量 0.4 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 APC-Cy7 (AQ870020)
反応性|交差吸着 Human | Non-Human Primates レーザー Red
抗原 CD11b, Mac-1 最大蛍光波長 780 nm
クローン ICRF44 最大励起波長 650 nm, 750 nm
ホスト Mouse 標識/Format APC-Cy7
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD11B APC-CY7,ICRF44

特異性

The mouse monoclonal antibody ICRF44 recognizes CD11b antigen, a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells.

抗原情報

CD11b (integrin αM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with CD18 (integrin β2 subunit); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Hua J, Sakamoto K, Nagaoka I: Inhibitory actions of glucosamine, a therapeutic agent for osteoarthritis, on the functions of neutrophils. J·Leukoc·Biol. 2002·Apr; 71(4):632‑40. <·PMID:·11927650·>
• Masten BJ, Olson GK, Tarleton CA, Rund C, Schuyler M, Mehran R, Archibeque T, Lipscomb MF: Characterization of myeloid and plasmacytoid dendritic cells in human lung. J·Immunol. 2006·Dec·1; 177(11):7784‑93. <·PMID:·17114449·>
• Rezzonico R, Imbert V, Chicheportiche R, Dayer JM: Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP‑1alpha) and MIP‑1beta production in primary human monocytes through a pathway dependent on nuclear factor‑kappaB. Blood. 2001·May·15; 97(10):2932‑40. <·PMID:·11342414·>
• Sánchez-Torres C, García-Romo GS, Cornejo-Cortés MA, Rivas-Carvalho A, Sánchez-Schmitz G: CD16+ and CD16‑ human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells. Int·Immunol. 2001·Dec; 13(12):1571‑81. <·PMID:·11717198·>
• Tieu BC, Lee C, Sun H, Lejeune W, Recinos A 3rd, Ju X, Spratt H, Guo DC, Milewicz D, Tilton RG, Brasier AR: An adventitial IL‑6/MCP1 amplification loop accelerates macrophage‑mediated vascular inflammation leading to aortic dissection in mice. J·Clin·Invest. 2009·Dec; 119(12):3637‑51. <·PMID:·19920349·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD11b APC-Cy7 (ICRF44) BE330247 TDS (US)