CyFlow CD95 FITC
| 品番 | BD855752 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD95 FITC,LT95 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 2 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 FITC (CD051682) |
||
| 反応性|交差吸着 | Human | レーザー | Blue |
| 抗原 | CD95, Fas, APO-1, TNFRSF6 | 最大蛍光波長 | 518 nm |
| クローン | LT95 | 最大励起波長 | 490 to 495 nm |
| ホスト | Mouse | 標識/Format | FITC |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping | Apoptosis |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD95 FITC,LT95
特異性
The mouse monoclonal antibody LT95 recognizes CD95 antigen, a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
抗原情報
CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Brumatti G, Yon M, Castro FA, Bueno-da-Silva AE, Jacysyn JF, Brunner T, Amarante-Mendes GP: Conversion of CD95 (Fas) Type II into Type I signaling by sub‑lethal doses of cycloheximide. Exp·Cell·Res. 2008·Feb·1; 314(3):554‑63. <·PMID:·18078929·>
• Drosopoulos KG, Roberts ML, Cermak L, Sasazuki T, Shirasawa S, Andera L, Pintzas A: Transformation by oncogenic RAS sensitizes human colon cells to TRAIL‑induced apoptosis by up‑regulating death receptor 4 and death receptor 5 through a MEK‑dependent pathway. J·Biol·Chem. 2005·Jun17; 280(24):22856‑67. <·PMID:·15757891·>
• Guo Z, Zhang M, Tang H, Cao X: Fas signal links innate and adaptive immunity by promoting dendritic‑cell secretion of CC and CXC chemokines. Blood. 2005·Sep·15; 106(6):2033‑41. <·PMID:·15941911·>
• Park DR, Thomsen AR, Frevert CW, Pham U, Skerrett SJ, Kiener PA, Liles WC: Fas (CD95 induces proinflammatory cytokine responses by human monocytes and monocyte‑derived macrophages. J·Immunol. 2003·Jun·15; 170(12):6209‑16. <·PMID:·12794152·>
• Scaffidi C, Fulda S, Srinivasan A, Friesen C, Li F, Tomaselli KJ, Debatin KM, Krammer PH, Peter ME: Two CD95 (APO‑1/Fas) signaling pathways. EMBO·J. 1998·Mar·16; 17(6):1675‑87. <·PMID:·9501089·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN