CyFlow CD62L PE
| 品番 | BC474812 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD62L PE,LT-TD180 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 2 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
||
| 反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
| 抗原 | CD62L, L-selectin | 最大蛍光波長 | 576 nm |
| クローン | LT-TD180 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD62L PE,LT-TD180
特異性
The mouse monoclonal antibody LT-TD180 recognizes CD62L antigen, a 74-95 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.
抗原情報
CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Ding Z, Issekutz TB, Downey GP, Waddell TK: L‑selectin stimulation enhances functional expression of surface CXCR4 in lymphocytes: implications for cellular activation during adhesion and migration. Blood. 2003·Jun·1; 101(11):4245‑52. <·PMID:·12609846·>
• Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A: NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood. 2009·Jan·8; 113(2):358‑69. <·PMID:·18922855·>
• Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI‑H tumor‑specific cytotoxic T cell epitope generated by the ‑1 frame of U79260(FTO). J·Biomed·Biotechnol. 2010; 2010:841451. <·PMID:·20339516·>
• Ramachandran V, Williams M, Yago T, Schmidtke DW, McEver RP: Dynamic alterations of membrane tethers stabilize leukocyte rolling on P‑selectin. Proc·Natl·Acad·Sci·USA. 2004·Sep·14; 101(37):13519‑24. <·PMID:·15353601·>
• Simon SI, Burns AR, Taylor AD, Gopalan PK, Lynam EB, Sklar LA, Smith CW: L‑selectin (CD62L) cross‑linking signals neutrophil adhesive functions via the Mac‑1 (CD11b/CD18 beta 2‑integrin. J·Immunol. 1995·Aug·1; 155(3):1502‑14. <·PMID:·7543524·>
• Simone R, Zicca A, Saverino D: The frequency of regulatory CD3+CD8+CD28‑ CD25+ T lymphocytes in human peripheral blood increases with age. J·Leukoc·Biol. 2008·Dec; 84(6):1454‑61. <·PMID:·18780874·>
• von Andrian UH, Chambers JD, Berg EL, Michie SA, Brown DA, Karolak D, Ramezani L, Berger EM, Arfors KE, Butcher EC: L‑selectin mediates neutrophil rolling in inflamed venules through sialyl LewisX‑dependent and ‑independent recognition pathways. Blood. 1993·Jul·1; 82(1):182‑91. <·PMID:·7686786·>
• Von Andrian UH, Hansell P, Chambers JD, Berger EM, Torres Filho I, Butcher EC, Arfors KE: L‑selectin function is required for beta 2‑integrin‑mediated neutrophil adhesion at physiological shear rates in vivo. Am·J·Physiol. 1992·Oct; 263(4‑2):H1034‑44. <·PMID:·1384360·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN