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CyFlow Notch 1 APC

品番 BB063499
抗体名 Anti-H/M NOTCH 1 APC,mN1A
包装単位 0.1 mg
濃度 0.1 mg/ml
容量 1 ml
関連製品
(アイソタイプコントロール)
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反応性|交差吸着 Human | Mouse レーザー Red
抗原 Notch 1 最大蛍光波長 660 nm
クローン mN1A 最大励起波長 650 nm
ホスト Mouse 標識/Format APC
アイソタイプ IgG1 研究分野 Transcription
クローナリティ monoclonal 用途

Flow cytometry

Anti-H/M NOTCH 1 APC,mN1A

特異性

The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.

抗原情報

Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged1 (CD339), and Jagged2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.

利用方法

The reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Espinosa L, Santos S, Inglés-Esteve J, Muñoz-Canoves P, Bigas A: p65‑NFkappaB synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear receptor corepressor N‑CoR. J·Cell·Sci. 2002·Mar·15; 115(6):1295‑303. <·PMID:·11884528·>
• Huppert SS, Le A, Schroeter EH, Mumm JS, Saxena MT, Milner LA, Kopan R: Embryonic lethality in mice homozygous for a processing‑deficient allele of Notch1. Nature. 2000·Jun·22; 405(6789):966‑70. <·PMID:·10879540·>
• Kefas B, Comeau L, Floyd DH, Seleverstov O, Godlewski J, Schmittgen T, Jiang J, diPierro CG, Li Y, Chiocca EA, Lee J, Fine H, Abounader R, Lawler S, Purow B: The neuronal microRNA miR‑326 acts in a feedback loop with notch and has therapeutic potential against brain tumors. J·Neurosci. 2009·Dec·2; 29(48):15161‑8. <·PMID:·19955368·>
• Khwaja SS, Liu H, Tong C, Jin F, Pear WS, van Deursen J, Bram RJ: HIV‑1 Rev‑binding protein accelerates cellular uptake of iron to drive Notch‑induced T cell leukemogenesis in mice. J·Clin·Invest. 2010·Jul·1; 120(7):2537‑48. <·PMID:·20516639·>
• Sun H, Li L, Vercherat C, Gulbagci NT, Acharjee S, Li J, Chung TK, Thin TH, Taneja R: Stra13 regulates satellite cell activation by antagonizing Notch signaling. J·Cell·Biol. 2007·May·21; 177(4):647‑57. <·PMID:·17502421·>
• Tagami S, Okochi M, Yanagida K, Ikuta A, Fukumori A, Matsumoto N, Ishizuka-Katsura Y, Nakayama T, Itoh N, Jiang J, Nishitomi K, Kamino K, Morihara T, Hashimoto R, Tanaka T, Kudo T, Chiba S, Takeda M: Regulation of Notch signaling by dynamic changes in the precision of S3 cleavage of Notch‑1. Mol·Cell·Biol. 2008·Jan; 28(1):165‑76. <·PMID:·17967888·>
• Watanabe K, Nagaoka T, Lee JM, Bianco C, Gonzales M, Castro NP, Rangel MC, Sakamoto K, Sun Y, Callahan R, Salomon DS: Enhancement of Notch receptor maturation and signaling sensitivity by Cripto‑1. J·Cell·Biol. 2009·Nov·2; 187(3):343‑53. <·PMID:·19948478·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ Notch 1 APC (mN1A) BB063499 TDS (US)