CyFlow CD8 PE-Cy7
品番 | BA413066 | ||
---|---|---|---|
抗体名 | Anti-Hu CD8 PE-CY7,MEM-31 | ||
包装単位 | 100 tests | ||
濃度 | - | ||
容量 | 0.4 ml | ||
関連製品 (アイソタイプコントロール) |
- | ||
反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
抗原 | CD8 | 最大蛍光波長 | 785 nm |
クローン | MEM-31 | 最大励起波長 | 496 nm, 565 nm |
ホスト | Mouse | 標識/Format | PE-Cy7 |
アイソタイプ | IgG2a | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD8 PE-CY7,MEM-31
特異性
The mouse monoclonal antibody MEM-31 recognizes a conformationally-dependent epitope of CD8 antigen, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 α/α homodimer or CD8 α/β heterodimer (each monomer approx. 32-34 kDa).
The antibody does not react with formaldehyde-fixed cells; negative in Western Blotting application.
抗原情報
The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as α/β heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as α/α homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 β level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 α-β but not α-α dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft‑associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J·Exp·Med. 2003·Nov·17; 198(10):1453‑62. <·PMID:·14610046·>
• Devine L, Thakral D, Nag S, Dobbins J, Hodsdon ME, Kavathas PB: Mapping the binding site on CD8 beta for MHC class I reveals mutants with enhanced binding. J·Immunol. 2006·Sep·15; 177(6):3930‑8. <·PMID:·16951356·>
• Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single‑molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int·Immunol. 2007·May; 19(5):675‑84. <·PMID:·17446208·>
• Estefanía E, Flores R, Gómez-Lozano N, Aguilar H, López-Botet M, Vilches C: Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets. J·Immunol. 2007·Apr·1; 178(7):4402‑10. <·PMID:·17371997·>
• Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I: Monoclonal antibodies against human leucocyte antigens (II); Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18‑kDa antigen (MEM‑43) and a novel antigen of restricted expression (MEM‑74). Folia·Biol·(Praha). 1988; 34(1):23‑34. <·PMID:·2968928·>
• Horejsi V, Hilgert I, Kristofova H, Bazil V, Bukovsky A, Kulhankova J: Monoclonal antibodies against human leucocyte antigens (I); Antibodies against beta‑2‑microglobulin, immunoglobulin kappa light chains, HLA‑DR‑like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan‑leucocyte antigen. Folia·Biol·(Praha). 1986; 32(1):12‑25. <·PMID:·2422063·>
• Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI‑H tumor‑specific cytotoxic T cell epitope generated by the ‑1 frame of U79260(FTO). J·Biomed·Biotechnol. 2010; 2010:841451. <·PMID:·20339516·>
• Pang DJ, Hayday AC, Bijlmakers MJ: CD8 Raft localization is induced by its assembly into CD8alpha beta heterodimers, Not CD8alpha alpha homodimers. J·Biol·Chem. 2007·May·4; 282(18):13884‑94. <·PMID:·17341584·>
• van den Berg HA, Wooldridge L, Laugel B, Sewell AK: Coreceptor CD8‑driven modulation of T cell antigen receptor specificity. J·Theor·Biol. 2007·Nov·21; 249(2):395‑408. <·PMID:·17869274·>
• McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN