特異性

The mouse monoclonal antibody MEM-259 recognizes CD63 antigen, a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.

抗原情報

CD63 (LAMP-3; lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Cerny J, Feng Y, Yu A, Miyake K, Borgonovo B, Klumperman J, Meldolesi J, McNeil PL, Kirchhausen T: The small chemical vacuolin‑1 inhibits Ca(2+)‑dependent lysosomal exocytosis but not cell resealing. EMBO·Rep. 2004·Sep; 5(9):883‑8. <·PMID:·15332114·>
• Grützkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM: LAMP‑1 and LAMP‑2, but not LAMP‑3, are reliable markers for activation‑induced secretion of human mast cells. Cytometry·A. 2004·Sep; 61(1):62‑8. <·PMID:·15351990·>
• Israels SJ, McMillan-Ward EM: CD63 modulates spreading and tyrosine phosphorylation of platelets on immobilized fibrinogen. Thromb·Haemost. 2005·Feb; 93(2):311‑8. <·PMID:·15711748·>
• Kwon MS, Shin SH, Yim SH, Lee KY, Kang HM, Kim TM, Chung YJ: CD63 as a biomarker for predicting the clinical outcomes in adenocarcinoma of lung. Lung·Cancer. 2007·Jul; 57(1):46‑53. <·PMID:·17350713·>
• Lin D, Kamsteeg EJ, Zhang Y, Jin Y, Sterling H, Yue P, Roos M, Duffield A, Spencer J, Caplan M, Wang WH: Expression of tetraspan protein CD63 activates protein tyrosine kinase (PTK) and enhances the PTK‑induced inhibition of ROMK channels. J·Biol·Chem. 2008·Jan·22; <·PMID:·19880522·>
• Mantegazza AR, Barrio MM, Moutel S, Bover L, Weck M, Brossart P, Teillaud JL, Mordoh J: CD63 tetraspanin slows down cell migration and translocates to theendosomal‑lysosomal‑MIICs route after extracellular stimuli in human immature dendritic cells. Blood. 2004·Aug·15; 104(4):1183‑90. <·PMID:·15130945·>
• Pfistershammer K, Majdic O, Stöckl J, Zlabinger G, Kirchberger S, Steinberger P, Knapp W: CD63 as an activation‑linked T cell costimulatory element. J·Immunol. 2004·Nov·15; 173(10):6000‑8. <·PMID:·15528334·>