特異性

The mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3 antigen, a 47-55 kDa transcription factor, which is the master regulator in the development and function of regulatory T cells.

抗原情報

FoxP3 (Forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and function of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

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• Bettini M, Vignali DA: Regulatory T cells and inhibitory cytokines in autoimmunity. Curr·Opin·Immunol. 2009·Dec; 21(6):612‑8. <·PMID:·19854631·>
• Elkord E: Novel therapeutic strategies by regulatory T cells in allergy. Chem·Immunol·Allergy. 2008; 94:150‑7. <·PMID:·18802345·>
• Gavin MA, Torgerson TR, Houston E, DeRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY: Single‑cell analysis of normal and FOXP3‑mutant human T cells: FOXP3 expression without regulatory T cell development. Proc·Natl·Acad·Sci·USA. 2006·Apr·25; 103(17):6659‑64. <·PMID:·16617117·>
• Kuhn A, Beissert S, Krammer PH: CD4(+)CD25 (+) regulatory T cells in human lupus erythematosus. Arch·Dermatol·Res. 2009·Jan; 301(1):71‑81. <·PMID:·18985367·>
• Lal G, Bromberg JS: Epigenetic mechanisms of regulation of Foxp3 expression. Blood. 2009·Oct·29; 114(18):3727‑35. <·PMID:·19641188·>
• Law JP, Hirschkorn DF, Owen RE, Biswas HH, Norris PJ, Lanteri MC: The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells. Cytometry·A. 2009·Dec; 75(12):1040‑50. <·PMID:·19845018·>