CyFlow CD162 FITC
| 品番 | AZ840692 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD162 FITC,TC2 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 2 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 FITC (CD051682) |
||
| 反応性|交差吸着 | Human | レーザー | Blue |
| 抗原 | CD162, PSGL1, PSGL-1 | 最大蛍光波長 | 518 nm |
| クローン | TC2 | 最大励起波長 | 490 to 495 nm |
| ホスト | Mouse | 標識/Format | FITC |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD162 FITC,TC2
特異性
The mouse monoclonal antibody TC2 recognizes CD162 antigen, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes; it is also expressed on a subpopulation of B lymphocytes and CD34+ bone marrow cells.
抗原情報
CD162 ( PSGL-1; P-selectin glycoprotein ligand-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Davenpeck KL, Brummet ME, Hudson SA, Mayer RJ, Bochner BS: Activation of human leukocyte reduces surface P‑selectin glycoprotein ligand‑1 (PSGL‑1, CD162 and adhesion to P‑selectin in vitro. J·Immunol. 2000·Sep·1; 165(5):2764‑72. <·PMID:·10946308·>
• Lévesque JP, Zannettino AC, Pudney M, Niutta S, Haylock DN, Snapp KR, Kansas GS, Berndt MC, Simmons PJ: PSGL‑1‑mediated adhesion of human hematopoietic progenitors to P‑selectin results in suppression of hematopoiesis. Immunity. 1999·Sep; 11(3):369‑78. <·PMID:·10514015·>
• Marsik C, Mayr F, Cardona F, Schaller G, Wagner OF, Jilma B: Endotoxin down‑modulates P‑selectin glycoprotein ligand‑1 (PSGL‑1, CD162) on neutrophils in humans. J·Clin·Immunol. 2004·Jan; 24(1):62‑5. <·PMID:·14997035·>
• Moore KL: Structure and function of P‑selectin glycoprotein ligand‑1. Leuk·Lymphoma. 1998·Mar; 29(1‑2):42005. <·PMID:·9638971·>
• Mason D, Simmons D, Buckley C, Schwartz-Albiez R, Hadam M, Saalmuller A, Clark E, Malavasi F, Morrissey JA (Eds): Leucocyte Typing VII. Oxford·University·Press. 2002; 1‑945. <·NLM·ID:·101177131·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN