研究用フローサイトと理化学分析

世界のリサーチ分野のお客さまへ先端の検査装置のご案内です。

お問い合わせはこちら

sysmex

研究用フローサイトと理化学分析sysmex

CyFlow CD4 FITC

品番 AZ405493
抗体名 Anti-Rt CD4 FITC,OX-35
包装単位 0.1 mg
濃度 0.5 mg/ml
容量 0.2 ml
関連製品
(アイソタイプコントロール)
Mouse IgG2a FITC (CZ676373)
反応性|交差吸着 Rat レーザー Blue
抗原 CD4, T4, Leu3a 最大蛍光波長 518 nm
クローン OX-35 最大励起波長 490 to 495 nm
ホスト Mouse 標識/Format FITC
アイソタイプ IgG2a 研究分野 Immunophenotyping | MHC
クローナリティ monoclonal 用途

Flow cytometry

Anti-Rt CD4 FITC,OX-35

特異性

The mouse monoclonal antibody OX-35 recognizes an extracellular epitope of rat CD4 transmembrane glycoprotein (55 kDa).

抗原情報

CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell differenciation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).

利用方法

The reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Baba T, Ishizu A, Iwasaki S, Suzuki A, Tomaru U, Ikeda H, Yoshiki T, Kasahara M: CD4+/CD8+ macrophages infiltrating at inflammatory sites: a population of monocytes/macrophages with a cytotoxic phenotype. Blood. 2006·Mar·1; 107(5):2004‑12. <·PMID:·16269616·>
• Baba T, Iwasaki S, Maruoka T, Suzuki A, Tomaru U, Ikeda H, Yoshiki T, Kasahara M, Ishizu A: Rat CD4+CD8+ macrophages kill tumor cells through an NKG2D‑ and granzyme/perforin‑dependent mechanism. J·Immunol. 2008·Mar·1; 180(5):2999‑3006. <·PMID:·18292522·>
• Dragun D, Lukitsch I, Tullius SG, Qun Y, Park JK, Schneider W, Luft FC, Haller H: Inhibition of intercellular adhesion molecule‑1 with antisense deoxynucleotides prolongs renal isograft survival in the rat. Kidney·Int. 1998·Dec; 54(6):2113‑22. <·PMID:·9853277·>
• Gelderman KA, Hultqvist M, Holmberg J, Olofsson P, Holmdahl R: T cell surface redox levels determine T cell reactivity and arthritis susceptibility. Proc·Natl·Acad·Sci·USA. 2006·Aug·22; 103(34):12831‑6. <·PMID:·16908843·>
• Hishikari K, Suzuki J, Ogawa M, Isobe K, Takahashi T, Onishi M, Takayama K, Isobe M: Pharmacological activation of the prostaglandin E2 receptor EP4 improves cardiacfunction after myocardial ischaemia/reperfusion injury. Cardiovasc·Res. 2009·Jan·1; 81(1):123‑32. <·PMID:·18805784·>
• Monzon-Casanova E, Steiniger B, Schweigle S, Clemen H, Zdzieblo D, Starick L, Müller I, Wang CR, Rhost S, Cardell S, Pyz E, Herrmann T: CD1d expression in paneth cells and rat exocrine pancreas revealed by novel monoclonal antibodies which differentially affect NKT cell activation. PLoS·One. 2010·Sep·30; 5(9):pii:e13089. <·PMID:·20927351·>
• Ramiro-Puig E, Pérez-Cano FJ, Ramos-Romero S, Pérez-Berezo T, Castellote C, Permanyer J, Franch A, Izquierdo-Pulido M, Castell M: Intestinal immune system of young rats influenced by cocoa‑enriched diet. J·Nutr·Biochem. 2008·Aug; 19(8):555‑65. <·PMID:·18061430·>
• Salomon I, Netzer N, Wildbaum G, Schif-Zuck S, Maor G, Karin N: Targeting the function of IFN‑gamma‑inducible protein 10 suppresses ongoing adjuvant arthritis. J·Immunol. 2002·Sep·1; 169(5):2685‑93. <·PMID:·12193742·>
• Tsuji H, Kawaguchi S, Wada T, Nagoya S, Inobe M, Yamashita T, Ishii S, Uede T: Adenovirus‑mediated in vivo B7‑1 gene transfer induces anti‑tumor immunity against pre‑established primary tumor and pulmonary metastasis of rat osteosarcoma. Cancer·Gene·Ther. 2002·Sep; 9(9):747‑55. <·PMID:·12189524·>
• Viel EC, Lemarié CA, Benkirane K, Paradis P, Schiffrin EL: Immune regulation and vascular inflammation in genetic hypertension. Am·J·Physiol·Heart·Circ·Physiol. 2010·Mar; 298(3):H938‑44. <·PMID:·20044442·>
• Yan Y, Devos T, Yu L, Xia G, Rutgeerts O, Goebels J, Segers C, Lin Y, Vandeputte M, Waer M: Pathogenesis of autoimmunity after xenogeneic thymus transplantation. J·Immunol. 2003·Jun·15; 170(12):5936‑46. <·PMID:·12794120·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD4 FITC (OX-35) AZ405493 TDS (US)