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CyFlow ZAP-70 Purified

品番 AY657586
抗体名 Anti-Hu ZAP-70 PURI,ZAP-03
包装単位 0.1 mg
濃度 1 mg/ml
容量 0.1 ml
関連製品
(アイソタイプコントロール)
-
反応性|交差吸着 Human レーザー
抗原 ZAP-70 最大蛍光波長 -
クローン ZAP-03 最大励起波長 -
ホスト Mouse 標識/Format Purified
アイソタイプ IgG1 研究分野 Phosphorylation
クローナリティ monoclonal 用途

Flow cytometry
Western blot
Immunocytochemistry

Anti-Hu ZAP-70 PURI,ZAP-03

特異性

The mouse monoclonal antibody ZAP-03 recognizes ZAP-70 antigen, a 70 kDa protein tyrosine kinase expressed in T and NK cells. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.

抗原情報

The ZAP-70; ζ-associated protein of 70 kDa tyrosine kinase, was identified as a tyrosine phosphoprotein that associates with TCR ζ subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP-70 gene mutations resulting in lower ZAP-70 protein expression levels or expression of catalytically inactive ZAP-70 proteins, have been identified. ZAP-70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency. In patients with chronic lymphocytic leukemia (B-CLL), ZAP-70 expression on B cell was shown to be correlated with disease progression and survival. ZAP-70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP-70 SH2 domains to the phosphorylated ζ subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP-70 on Tyr493 in the activation loop. ZAP-70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCγ1, Vav, Shc, Ras-GAP, and Abl. ZAP-70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Chan AC, Irving BA, Fraser JD, Weiss A: The zeta chain is associated with a tyrosine kinase and upon T‑cell antigen receptor stimulation associates with ZAP‑70, a 70‑kDa tyrosine phosphoprotein. Proc·Natl·Acad·Sci·USA. 1991·Oct·15; 88(20):9166‑70. <·PMID:·1717999·>
• Hrdinka M, Dráber P, Stepánek O, Ormsby T, Otáhal P, Angelisová P, Brdicka T, Paces J, Horejsí V, Drbal K: PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis. J·Biol·Chem. 2011·Jun·3; 286(22):19617‑29. <·PMID:·21460222·>
• Ishaq M, DeGray G, Natarajan V: Evidence for the involvement of tyrosine kinase ZAP 70 in nuclear retinoid receptor‑dependent transactivation in T lymphocytes. J·Biol·Chem. 2005·Oct·7; 280(40):34152‑8. <·PMID:·16096284·>
• Schneider H, Smith X, Liu H, Bismuth G, Rudd CE: CTLA‑4 disrupts ZAP70 microcluster formation with reduced T cell/APC dwell times and calcium mobilization. Eur·J·Immunol. 2008·Jan; 38(1):40‑7. <·PMID:·18095376·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ ZAP-70 Purified (ZAP-03) AY657586 TDS (US)