CyFlow CD62L FITC
品番 | AX041427 | ||
---|---|---|---|
抗体名 | Anti-Ms CD62L FITC,MEL-14 | ||
包装単位 | 0.1 mg | ||
濃度 | 0.5 mg/ml | ||
容量 | 0.2 ml | ||
関連製品 (アイソタイプコントロール) |
- | ||
反応性|交差吸着 | Mouse | レーザー | Blue |
抗原 | CD62L, L-selectin | 最大蛍光波長 | 518 nm |
クローン | MEL-14 | 最大励起波長 | 490 to 495 nm |
ホスト | Rat | 標識/Format | FITC |
アイソタイプ | IgG2a | 研究分野 | Immunophenotyping | MHC |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Ms CD62L FITC,MEL-14
特異性
The rat monoclonal antibody MEL-14 recognizes mouse CD62L, a 75 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.
抗原情報
CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).
利用方法
The reagent is designed for Flow Cytometry analysis. Suggested working usage is 6 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Brawand P, Cerottini JC, MacDonald HR: Hierarchal utilization of different T‑cell receptor Vbeta gene segments in the CD8(+)‑T‑cell response to an immunodominant Moloney leukemia virus‑encoded epitope in vivo. J·Virol. 1999·Nov; 73(11):9161‑9. <·PMID:·10516023·>
• Chen BJ, Cui X, Sempowski GD, Liu C, Chao NJ: Transfer of allogeneic CD62L‑ memory T cells without graft‑versus‑host disease. Blood. 2004·Feb·15; 103(4):1534‑41. <·PMID:·14551132·>
• Chin CS, Miller CH, Graham L, Parviz M, Zacur S, Patel B, Duong A, Bear HD: Bryostatin 1/ionomycin (B/I) ex vivo stimulation preferentially activates L‑selectinlow tumor‑sensitized lymphocytes. Int·Immunol. 2004·Sep; 16(9):1283‑94. <·PMID:·15262898·>
• Fisson S, Darrasse-Jèze G, Litvinova E, Septier F, Klatzmann D, Liblau R, Salomon BL: Continuous activation of autoreactive CD4+ CD25+ regulatory T cells in the steady state. J·Exp·Med. 2003·Sep·1; 198(5):737‑46. <·PMID:·12939344·>
• Friedline RH, Wong CP, Steeber DA, Tedder TF, Tisch R: L‑selectin is not required for T cell‑mediated autoimmune diabetes. J·Immunol. 2002·Mar·15; 168(6):2659‑66. <·PMID:·11884430·>
• Gallatin WM, Weissman IL, Butcher EC: A cell‑surface molecule involved in organ‑specific homing of lymphocytes. Nature. 1983·Jul·7; 304:30‑34. <·PMID:·6866086·>
• Mannering SI, Cheers C: Interleukin‑2 and loss of immunity in experimental Mycobacterium avium infection. Infect·Immun. 2002·Jan; 70(1):27‑35. <·PMID:·11748160·>
• Pop SM, Wong CP, He Q, Wang Y, Wallet MA, Goudy KS, Tisch R: The type and frequency of immunoregulatory CD4+ T‑cells govern the efficacy of antigen‑specific immunotherapy in nonobese diabetic mice. Diabetes. 2007·May; 56(5):1395‑402. <·PMID:·17317763·>
• Richards H, Longhi MP, Wright K, Gallimore A, Ager A: CD62L (L‑selectin) down‑regulation does not affect memory T cell distribution but failure to shed compromises anti‑viral immunity. J·Immunol. 2008·Jan·1; 180(1):198‑206. <·PMID:·18097020·>
• ten Bruggencate SJ, Hillyer LM, Woodward BD: The proportion of CD45RA(+)CD62L(+) (quiescent‑phenotype) T cells within the CD8(+) subset increases in advanced weight loss in the protein‑ or energy‑deficient weanling mouse. J·Nutr. 2001·Dec; 131(12):3266‑9. <·PMID:·11739878·>
• Xu H, Manivannan A, Crane I, Dawson R, Liversidge J: Critical but divergent roles for CD62L and CD44 in directing blood monocyte trafficking in vivo during inflammation. Blood. 2008·Aug·15; 112(4):1166‑74. <·PMID:·18391078·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN