特異性

The mouse monoclonal antibody UT-1 recognizes CD95 antigen, a 46 kDa glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. The antibody UT-1 does excellently induce Fas mediated apoptosis, similarly as CH11 antibody.

抗原情報

CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Brumatti G, Yon M, Castro FA, Bueno-da-Silva AE, Jacysyn JF, Brunner T, Amarante-Mendes GP: Conversion of CD95 (Fas) Type II into Type I signaling by sub‑lethal doses of cycloheximide. Exp·Cell·Res. 2008·Feb·1; 314(3):554‑63. <·PMID:·18078929·>
• Guo Z, Zhang M, Tang H, Cao X: Fas signal links innate and adaptive immunity by promoting dendritic‑cell secretion of CC and CXC chemokines. Blood. 2005·Sep·15; 106(6):2033‑41. <·PMID:·15941911·>
• Matsuyama W, Mitsuyama H, Ono M, Shirahama Y, Higashimoto I, Osame M, Arimura K: Discoidin domain receptor 1 contributes to eosinophil survival in an NF‑kappaB‑dependent manner in Churg‑Strauss syndrome. Blood. 2007·Jan·1; 109(1):22‑30. <·PMID:·18299452·>
• Park DR, Thomsen AR, Frevert CW, Pham U, Skerrett SJ, Kiener PA, Liles WC: Fas (CD95 induces proinflammatory cytokine responses by human monocytes and monocyte‑derived macrophages. J·Immunol. 2003·Jun·15; 170(12):6209‑16. <·PMID:·12794152·>
• Scaffidi C, Fulda S, Srinivasan A, Friesen C, Li F, Tomaselli KJ, Debatin KM, Krammer PH, Peter ME: Two CD95 (APO‑1/Fas) signaling pathways. EMBO·J. 1998·Mar·16; 17(6):1675‑87. <·PMID:·9501089·>