CyFlow CD3 PerCP-Cy5.5
| 品番 | AV981175 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD3 PERCP-CY5.5,SK7 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 0.4 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 PerCP-Cy5.5 (CZ886982) |
||
| 反応性|交差吸着 | Human | Non-Human Primates | レーザー | Blue, Green |
| 抗原 | CD3, T3, Leu4 | 最大蛍光波長 | 695 nm |
| クローン | SK7 | 最大励起波長 | 482 nm |
| ホスト | Mouse | 標識/Format | PerCP-Cy5.5 |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD3 PERCP-CY5.5,SK7
特異性
The mouse monoclonal antibody SK7 recognizes CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody reacts with the ε chain of the CD3 complex. The monoclonal antibodies SK7 and UCHT1 recognize overlapping epitopes.
抗原情報
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3 δ, CD3ε and CD3ζ. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Guttman-Yassky E, Vugmeyster Y, Lowes MA, Chamian F, Kikuchi T, Kagen M, Gilleaudeau P, Lee E, Hunte B, Howell K, Dummer W, Bodary SC, Krueger JG: Blockade of CD11a by efalizumab in psoriasis patients induces a unique state of T‑cell hyporesponsiveness. J·Invest·Dermatol. 2008·May; 128(5):1182‑91. <·PMID:·18239614·>
• Li B, Wang H, Dai J, Ji J, Qian W, Zhang D, Hou S, Guo Y: Construction and characterization of a humanized anti‑human CD3 monoclonal antibody 12F6 with effective immunoregulation functions. Immunology. 2005·Dec; 116(4):487‑98. <·PMID:·16313362·>
• Recio MJ, Moreno-Pelayo MA, Kiliç SS, Guardo AC, Sanal O, Allende LM, Pérez-Flores V, Mencía A, Modamio-Høybjør S, Seoane E, Regueiro JR: Differential biological role of CD3 chains revealed by human immunodeficiencies. J·Immunol. 2007·Feb·15; 178(4):2556‑64. <·PMID:·17277165·>
• Wood GS, Burns BF, Dorfman RF, Warnke RA: The immunohistology of non‑T cells in the acquired immunodeficiency syndrome. Am·J·Pathol. 1985·Sep; 120(3):371‑9. <·PMID:·3898858·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN