CyFlow CD47 Purified
品番 | AV372758 | ||
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抗体名 | Anti-Hu CD47 PURI,MEM-122 | ||
包装単位 | 0.1 mg | ||
濃度 | 1 mg/ml | ||
容量 | 0.1 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgM Purified (AP063643) |
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反応性|交差吸着 | Human | Non-Human Primates | Pig | レーザー | |
抗原 | CD47, IAP | 最大蛍光波長 | - |
クローン | MEM-122 | 最大励起波長 | - |
ホスト | Mouse | 標識/Format | Purified |
アイソタイプ | IgM | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD47 PURI,MEM-122
特異性
The mouse monoclonal antibody MEM-122 recognizes CD47 antigen, a 50-55 kDa membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.
抗原情報
CD47 (IAP; integrin-associated protein) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (SIRP α; signal regulatory protein α), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
利用方法
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Barazi HO, Li Z, Cashel JA, Krutzsch HC, Annis DS, Mosher DF, Roberts DD: Regulation of integrin function by CD47 ligands: Differential effects on alphavbeta 3 and alpha 4beta1 integrin‑mediated adhesion. J·Biol·Chem. 2002·Nov·8; 277(45):42859‑66. <·PMID:·12218055·>
• Ide K, Wang H, Tahara H, Liu J, Wang X, Asahara T, Sykes M, Yang YG, Ohdan H: Role for CD47‑SIRPalpha signaling in xenograft rejection by macrophages. Proc·Natl·Acad·Sci·USA. 2007·Mar·20; 104(12):5062‑6. <·PMID:·17360380·>
• Isenberg JS, Pappan LK, Romeo MJ, Abu-Asab M, Tsokos M, Wink DA, Frazier WA, Roberts DD: Blockade of thrombospondin‑1‑CD47 interactions prevents necrosis of full thickness skin grafts. Ann·Surg. 2008·Jan; 247(1):180‑90. <·PMID:·18156939·>
• Murata T, Ohnishi H, Okazawa H, Murata Y, Kusakari S, Hayashi Y, Miyashita M, Itoh H, Oldenborg PA, Furuya N, Matozaki T: CD47 promotes neuronal development through Src‑ and FRG/Vav2‑mediated activation of Rac and Cdc42. J·Neurosci. 2006·Nov·29; 26(48):12397‑407. <·PMID:·17135401·>
• Orazizadeh M, Lee HS, Groenendijk BC, Millward-Sadler J, Wright MO, Lindberg FP, Salter DM: CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model. Arthritis·Res·Ther. 2008·Jan·10; 10(1):R4. <·PMID:·18186923·>
• Smith RE, Patel V, Seatter SD, Deehan MR, Brown MH, Brooke GP, Goodridge HS, Howard CJ, Rigley KP, Harnett W, Harnett MM: A novel MyD‑1 (SIRP‑1alpha) signaling pathway that inhibits LPS‑induced TNFalpha production by monocytes. Blood. 2003·Oct·1; 102(7):2532‑40. <·PMID:·12805067·>
• Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN