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CyFlow IgG2a Pacific Blue

品番 AU451907
抗体名 Mouse IGG2A PAC BLUE,PPV-04
包装単位 0.1 mg
濃度 1 mg/ml
容量 0.1 ml
関連製品
(アイソタイプコントロール)
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反応性|交差吸着 レーザー Violet
抗原 IgG2a Isotype Control 最大蛍光波長 455 nm
クローン PPV-04 最大励起波長 400 to 410 nm
ホスト Mouse 標識/Format Pacific Blue™
アイソタイプ IgG2a 研究分野 Isotype controls
クローナリティ monoclonal 用途

Flow cytometry (Isotype control)

Mouse IGG2A PAC BLUE,PPV-04

特異性

This mouse IgG2a monoclonal antibody (clone PPV-04) reacts with undefined epitope on a plant pathogen.

抗原情報

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15μ/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-04, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

利用方法

The reagent is designed as an isotype control for Flow Cytometry analysis. To establish the amount of non-specific antibody binding, match the concentration of the correct isotype to the recommended working concentration of the antigen-specific antibody. If the background signal of the isotype control is too high (usually when working antibody concentrations are above 10 µg/ml of incubation mixture), change the experimental conditions to reduce the background.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

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• Vogel K, Thomann S, Vogel B, Schuster P, Schmidt B: Both plasmacytoid dendritic cells and monocytes stimulate natural killer cells early during human herpes simplex virus type 1 infections. Immunology. 2014·Dec; 143(4):588-600. <·PMID:·24943264·>
• Thomann S, Boscheinen JB, Vogel K, Knipe DM, DeLuca N, Gross S, Schuler-Thurner B, Schuster P, Schmidt B: Combined cytotoxic activity of an infectious, but non‐replicative herpes simplex virus type 1 and plasmacytoid dendritic cells against tumour cells. Immunology. 2015·Oct; 146(2):327-38. <·PMID:·26194553·>