CyFlow CD53 PE
| 品番 | AU131901 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD53 PE,MEM-53 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 2 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
||
| 反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
| 抗原 | CD53, TSPAN2 | 最大蛍光波長 | 576 nm |
| クローン | MEM-53 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD53 PE,MEM-53
特異性
The mouse monoclonal antibody MEM-53 recognizes CD53 antigen, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
抗原情報
CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumor cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Angelisová P, Vlcek C, Stefanova I, Lipoldova M, Horejsi V: The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX‑44 antigens.. Immunogenetics. 1990; 32(4):281‑5. <·PMID:·1700763·>
• Bazil V, Horejsi V, Baudys M, Kristofova H, Strominger JL, Kostka W, Hilgert I: Biochemical characterization of a soluble form of the 53‑kDa monocyte surface antigen. Eur·J·Immunol. 1986·Dec; 16(12):1583‑9. <·PMID:·3493149·>
• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Kim TR, Yoon JH, Kim YC, Yook YH, Kim IG, Kim YS, Lee H, Paik SG: LPS‑induced CD53 expression: a protection mechanism against oxidative and radiation stress. Mol·Cells. 2004·Feb·29; 17(1):125‑31. <·PMID:·15055538·>
• Mollinedo F, Martín-Martín B, Gajate C, Lazo PA: Physiological activation of human neutrophils down‑regulates CD53 cell surface antigen. J·Leukoc·Biol. 1998·Jun; 63(6):699‑706. <·PMID:·9620662·>
• Olweus J, Lund-Johansen F, Horejsi V: CD53, a protein with four membrane‑spanning domains, mediates signal transduction in human monocytes and B cells. J·Immunol. 1993·Jul·15; 151(2):707‑16. <·PMID:·8335905·>
• Olweus J, Lund-Johansen F, Horejsi V: CD53, a protein with four membrane‑spanning domains, mediates signal transduction in human monocytes and B cells. J·Immunol. 1993·Jul·15; 151(2):707‑16. <·PMID:·8335905·>
• Puls KL, Hogquist KA, Reilly N, Wright MD: CD53, a thymocyte selection marker whose induction requires a lower affinity TCR‑MHC interaction than CD69, but is up‑regulated with slower kinetics. Int·Immunol. 2002·Mar; 14(3):249‑58. <·PMID:·11867561·>
• Rasmussen AM, Blomhoff HK, Stokke T, Horejsi V, Smeland EB: Cross‑linking of CD53 promotes activation of resting human B lymphocytes. J·Immunol. 1994·Dec·1; 153(11):4997‑5007. <·PMID:·7963560·>
• Szollosi J, Horejsi V, Bene L, Angelisova P, Damjanovich S: Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. J·Immunol. 1996·Oct·1; 157(7):2939‑46. <·PMID:·8816400·>
• Yunta M, Lazo PA: Apoptosis protection and survival signal by the CD53 tetraspanin antigen. Oncogene. 2003·Feb·27; 22(8):1219‑24. <·PMID:·12606948·>
• Yunta M, Rodríguez-Barbero A, Arévalo MA, López-Novoa JM, Lazo PA: Induction of DNA synthesis by ligation of the CD53 tetraspanin antigen in primarycultures of mesangial cells. Kidney·Int. 2003·Feb; 63(2):534‑42. <·PMID:·12631118·>
• Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN