特異性

The mouse monoclonal antibody OX-35 recognizes an extracellular epitope of rat CD4 transmembrane glycoprotein (55 kDa).

抗原情報

CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell differenciation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Baba T, Ishizu A, Iwasaki S, Suzuki A, Tomaru U, Ikeda H, Yoshiki T, Kasahara M: CD4+/CD8+ macrophages infiltrating at inflammatory sites: a population of monocytes/macrophages with a cytotoxic phenotype. Blood. 2006·Mar·1; 107(5):2004‑12. <·PMID:·16269616·>
• Baba T, Iwasaki S, Maruoka T, Suzuki A, Tomaru U, Ikeda H, Yoshiki T, Kasahara M, Ishizu A: Rat CD4+CD8+ macrophages kill tumor cells through an NKG2D‑ and granzyme/perforin‑dependent mechanism. J·Immunol. 2008·Mar·1; 180(5):2999‑3006. <·PMID:·18292522·>
• Dragun D, Lukitsch I, Tullius SG, Qun Y, Park JK, Schneider W, Luft FC, Haller H: Inhibition of intercellular adhesion molecule‑1 with antisense deoxynucleotides prolongs renal isograft survival in the rat. Kidney·Int. 1998·Dec; 54(6):2113‑22. <·PMID:·9853277·>
• Gelderman KA, Hultqvist M, Holmberg J, Olofsson P, Holmdahl R: T cell surface redox levels determine T cell reactivity and arthritis susceptibility. Proc·Natl·Acad·Sci·USA. 2006·Aug·22; 103(34):12831‑6. <·PMID:·16908843·>
• Hishikari K, Suzuki J, Ogawa M, Isobe K, Takahashi T, Onishi M, Takayama K, Isobe M: Pharmacological activation of the prostaglandin E2 receptor EP4 improves cardiacfunction after myocardial ischaemia/reperfusion injury. Cardiovasc·Res. 2009·Jan·1; 81(1):123‑32. <·PMID:·18805784·>
• Monzon-Casanova E, Steiniger B, Schweigle S, Clemen H, Zdzieblo D, Starick L, Müller I, Wang CR, Rhost S, Cardell S, Pyz E, Herrmann T: CD1d expression in paneth cells and rat exocrine pancreas revealed by novel monoclonal antibodies which differentially affect NKT cell activation. PLoS·One. 2010·Sep·30; 5(9):pii:e13089. <·PMID:·20927351·>
• Ramiro-Puig E, Pérez-Cano FJ, Ramos-Romero S, Pérez-Berezo T, Castellote C, Permanyer J, Franch A, Izquierdo-Pulido M, Castell M: Intestinal immune system of young rats influenced by cocoa‑enriched diet. J·Nutr·Biochem. 2008·Aug; 19(8):555‑65. <·PMID:·18061430·>
• Salomon I, Netzer N, Wildbaum G, Schif-Zuck S, Maor G, Karin N: Targeting the function of IFN‑gamma‑inducible protein 10 suppresses ongoing adjuvant arthritis. J·Immunol. 2002·Sep·1; 169(5):2685‑93. <·PMID:·12193742·>
• Tsuji H, Kawaguchi S, Wada T, Nagoya S, Inobe M, Yamashita T, Ishii S, Uede T: Adenovirus‑mediated in vivo B7‑1 gene transfer induces anti‑tumor immunity against pre‑established primary tumor and pulmonary metastasis of rat osteosarcoma. Cancer·Gene·Ther. 2002·Sep; 9(9):747‑55. <·PMID:·12189524·>
• Viel EC, Lemarié CA, Benkirane K, Paradis P, Schiffrin EL: Immune regulation and vascular inflammation in genetic hypertension. Am·J·Physiol·Heart·Circ·Physiol. 2010·Mar; 298(3):H938‑44. <·PMID:·20044442·>
• Yan Y, Devos T, Yu L, Xia G, Rutgeerts O, Goebels J, Segers C, Lin Y, Vandeputte M, Waer M: Pathogenesis of autoimmunity after xenogeneic thymus transplantation. J·Immunol. 2003·Jun·15; 170(12):5936‑46. <·PMID:·12794120·>