CyFlow CD18 Biotin
| 品番 | AT212653 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD18 BIOTIN,MEM-48 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 1 mg/ml | ||
| 容量 | 0.1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 Biotin (AQ807476) |
||
| 反応性|交差吸着 | Human | レーザー | |
| 抗原 | CD18, b2 integrin | 最大蛍光波長 | - |
| クローン | MEM-48 | 最大励起波長 | - |
| ホスト | Mouse | 標識/Format | Biotin |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD18 BIOTIN,MEM-48
特異性
The mouse monoclonal antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen. CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
抗原情報
CD18 (integrin β2 subunit) forms heterodimers with four types of CD11 molecule to constitute leukocyte (β2) integrins: αLβ2 (CD11a/CD18, LFA-1), αMβ2 (CD11b/CD18, Mac-1, CR3), αXβ2 (CD11c/CD18) and αDβ2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
利用方法
The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 10 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
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• Dao MA, Arevalo J, Nolta JA: Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution. Blood. 2003·Jan·1; 101(1):112‑8. <·PMID:·12589631·>
• Gannon GA, Rhind SG, Suzui M, Zamecnik J, Sabiston BH, Shek PN, Shephard RJ: beta‑Endorphin and natural killer cell cytolytic activity during prolonged exercise: Is there a connection?. Am·J·Physiol. 1998·Dec; 275(6‑2):R1725‑34. <·PMID:·9843861·>
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• Kerstan A, Hünig T: Cutting edge: distinct TCR‑ and CD28‑derived signals regulate CD95L, Bcl‑xL, and the survival of primary T cells. J·Immunol. 2004·Feb·1; 172(3):1341‑5. <·PMID:·14734708·>
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• Larson RS, Springer TA: Structure and function of leukocyte integrins. Immunol·Rev. 1990·Apr; 114:181‑217. <·PMID:·2196220·>
• Ottonello L, Epstein AL, Dapino P, Barbera P, Morone P, Dallegri F: Monoclonal Lym‑1 antibody‑dependent cytolysis by neutrophils exposed to granulocyte‑macrophage colony‑stimulating factor: intervention of FcgammaRII (CD32), CD11b‑CD18 integrins, and CD66b glycoproteins. Blood. 1999·May·15; 93(10):3505‑11. <·PMID:·10233903·>
• Persson H, Hennighausen L, Taub R, DeGrado W, Leder P: Antibodies to human c‑myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation. Science. 1984·Aug·17; 225(4663):687‑93. <·PMID:·6431612·>
• Schiff DE, Rae J, Martin TR, Davis BH, Curnutte JT: Increased phagocyte Fc gammaRI expression and improved Fc gamma‑receptor‑mediated phagocytosis after in vivo recombinant human interferon‑gamma treatment of normal human subjects. Blood. 1997·Oct·15; 90(8):3187‑94. <·PMID:·9376602·>
• Shan M, Klasse PJ, Banerjee K, Dey AK, Iyer SP, Dionisio R, Charles D, Campbell-Gardener L, Olson WC, Sanders RW, Moore JP: HIV‑1 gp120 mannoses induce immunosuppressive responses from dendritic cells. PLoS·Pathog. 2007·Nov; 3(11):e169. <·PMID:·17983270·>
• Solovey AN, Gui L, Chang L, Enenstein J, Browne PV, Hebbel RP: Identification and functional assessment of endothelial P1H12. J·Lab·Clin·Med. 2001·Nov; 138(5):322‑31. <·PMID:·11709656·>
• Stefanova I, Horejsi V: Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules. J·Immunol. 1991·Sep·1; 147(5):1587‑92. <·PMID:·1715364·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN