CyFlow CD18 PE
| 品番 | AS957495 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD18 PE,MEM-148 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 2 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
||
| 反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
| 抗原 | CD18, b2 integrin | 最大蛍光波長 | 576 nm |
| クローン | MEM-148 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD18 PE,MEM-148
特異性
The mouse monoclonal antibody MEM-148 recognizes an epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
抗原情報
CD18 (integrin β2 subunit) forms heterodimers with four types of CD11 molecule to constitute leukocyte (β2) integrins: αLβ2 (CD11a/CD18, LFA-1), αMβ2 (CD11b/CD18, Mac-1, CR3), αXβ2 (CD11c/CD18) and αDβ2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
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• Dassanayake RP, Maheswaran SK, Srikumaran S: Monomeric expression of bovine beta2‑integrin subunits reveals their role in Mannheimia haemolytica leukotoxin‑induced biological effects. Infect·Immun. 2007·Oct; 75(10):5004‑10. <·PMID:·17698568·>
• Drbal K, Angelisova P, Cerny J, Hilgert I, Horejsi V: A novel anti‑CD18 mAb recognizes an activation‑related epitope and induces a high‑affinity conformation in leukocyte integrins. Immunobiology. 2001·May; 203(4):687‑98. <·PMID:·11402502·>
• Drbal K, Angelisova P, Cerny J, Pavlistova D, Cebecauer M, Novak P, Horejsi V: Human leukocytes contain a large pool of free forms of CD18. Biochem·Biophys·Res·Commun. 2000·Aug·28; 275(2):295‑9. <·PMID:·10964661·>
• Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001·Sep·1; 98(5):1561‑6. <·PMID:·11520808·>
• Gao JX, Issekutz AC: Mac‑1 (CD11b/CD108) is the predominant beta 2 (CD108) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers. Immunology. 1996·Jul; 88(3):463‑70. <·PMID:·8774366·>
• Kess D, Peters T, Zamek J, Wickenhauser C, Tawadros S, Loser K, Varga G, Grabbe S, Nischt R, Sunderkötter C, Müller W, Krieg T, Scharffetter-Kochanek K: CD4+ T cell‑associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis. J·Immunol. 2003·Dec·1; 171(11):5697‑706. <·PMID:·14634077·>
• Peters T, Sindrilaru A, Wang H, Oreshkova T, Renkl AC, Kess D, Scharffetter-Kochanek K: CD18 in monogenic and polygenic inflammatory processes of the skin. J·Investig·Dermatol·Symp·Proc. 2006·Sep; 11(1):42186. <·PMID:·17069006·>
• Shang XZ, Issekutz AC: Contribution of CD11a/CD18, CD11b/CD18, ICAM‑1 (CD54 and ‑2 (CD102 to human monocyte migration through endothelium and connective tissue fibroblast barriers. Eur·J·Immunol. 1998·Jun; 28(6):1970‑9. <·PMID:·9645379·>
• Solovjov DA, Pluskota E, Plow EF: Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2. J·Biol·Chem. 2005·Jan·14; 280(2):1336‑45. <·PMID:·15485828·>
• Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN