CyFlow IgG1 Purified
| 品番 | AS224818 | ||
|---|---|---|---|
| 抗体名 | Mouse IGG1 PURI,PPV-06 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 1 mg/ml | ||
| 容量 | 0.1 ml | ||
| 関連製品 (アイソタイプコントロール) |
- | ||
| 反応性|交差吸着 | レーザー | ||
| 抗原 | IgG1 Isotype Control | 最大蛍光波長 | - |
| クローン | PPV-06 | 最大励起波長 | - |
| ホスト | Mouse | 標識/Format | Purified |
| アイソタイプ | IgG1 | 研究分野 | Isotype controls |
| クローナリティ | monoclonal | 用途 | Flow cytometry (Isotype control) |
Mouse IGG1 PURI,PPV-06
特異性
This mouse IgG1 monoclonal antibody (clone PPV-06) reacts with undefined epitope on a plant pathogen.
抗原情報
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-06, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
利用方法
The reagent is designed as an isotype control for Flow Cytometry analysis. To establish the amount of non-specific antibody binding, match the concentration of the correct isotype to the recommended working concentration of the antigen-specific antibody. If the background signal of the isotype control is too high (usually when working antibody concentrations are above 10 µg/ml of incubation mixture), change the experimental conditions to reduce the background.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Larsen MH, Bzorek M, Pass MB, Larsen LG, Nielsen MW, Svendsen SG, Lindhard A, Hviid TV: Human leukocyte antigen‑G in the male reproductive system and in seminal plasma. Mol·Hum·Reprod. 2011·Dec; 17(12):727‑38. <·PMID:·21813635·>
• Mehling M, Burgener AV, Brinkmann V, Bantug GR, Dimeloe S, Hoenger G, Kappos L, Hess C: Tissue Distribution Dynamics of Human NK Cells Inferred from Peripheral Blood Depletion Kinetics after Sphingosine-1-Phosphate Receptor Blockade.. Scand·J·Immunol. 2015·Nov; 82(5):460-6. <·PMID:·26285786·>
• Almeida CR, Vasconcelos DP, Gonçalves RM, Barbosa MA: Enhanced mesenchymal stromal cell recruitment via natural killer cells by incorporation of inflammatory signals in biomaterials. J·R·Soc·Interface. 2012·Feb·7; 9(67):261-71. <·PMID:·21752807·>
• Svendsen SG, Hantash BM, Zhao L, Faber C, Bzorek M, Nissen MH, Hviid TV: The expression and functional activity of membrane-bound human leukocyte antigen-G1 are influenced by the 3′-untranslated region. Hum·Immunol. 2013·Jul; 74(7):818-27. <·PMID:·23583363·>
• Handke W, Oelschlegel R, Franke R, Krüger DH, Rang A: Hantaan virus triggers TLR3-dependent innate immune responses. J·Immunol. 2009·Mar·1; 182(5):2849-58. <·PMID:·19234180·>
• Michaelis K, Wallbrecht K, Kerstan A, Beyersdorf N, Williams C, Kerkau T, Wang XJ, Hünig T, Schön MP: Modulating T cell functions does not alleviate chronic inflammatory skin lesions in K5.TGF beta 1 transgenic mice.. Exp·Dermatol. 2010·May; 19(5):406-15. <·PMID:·20100197·>
• Beyersdorf N, Ding X, Blank G, Dennehy KM, Kerkau T, Hünig T: Protection from graft-versus-host disease with a novel B7 binding site–specific mouse anti–mouse CD28 monoclonal antibody. Blood. 2008·Nov·15; 112(10):4328-36. <·PMID:·18780833·>
• Balogh A, Adori M, Török K, Matko J, László G: A closer look into the GL7 antigen: its spatio-temporally selective differential expression and localization in lymphoid cells and organs in human. Immunol·Lett. 2010·May·4; 130(1-2):89-96. <·PMID:·20005896·>
• Escate R, Padro T, Badimon L: LDL accelerates monocyte to macrophage differentiation: Effects on adhesion and anoikis. Atherosclerosis. 2016·Mar; 246:177-86. <·PMID:·26800307·>
• Szittner Z, Papp K, Sándor N, Bajtay Z, Prechl J: Application of fluorescent monocytes for probing immune complexes on antigen microarrays. PLoS·One. 2013·Sep·5; 8(9):e72401. <·PMID:·24039758·>
• Correia MP, Costa AV, Uhrberg M, Cardoso EM, Arosa FA: IL-15 induces CD8+ T cells to acquire functional NK receptors capable of modulating cytotoxicity and cytokine secretion. Immunobiology. 2011·May; 216(5):604-12. <·PMID:·20956026·>
• Beyersdorf N, Werner S, Wolf N, Herrmann T, Kerkau T: Characterization of a new mouse model for peripheral T cell lymphoma in humans. PLoS·One. 2011; 6(12):e28546. <·PMID:·22163033·>
• Schmidt T, Willenborg S, Hünig T, Deeg CA, Sonderstrup G, Hertl M, Eming R: Induction of T regulatory cells by the superagonistic anti-CD28 antibody D665 leads to decreased pathogenic IgG autoantibodies against desmoglein 3 in a HLA-transgenic mouse model of pemphigus vulgaris.. Exp·Dermatol. 2016·Apr; 25(4):293-8. <·PMID:·26661498·>
• van Bergenhenegouwen J, Kraneveld AD, Rutten L, Kettelarij N, Garssen J, Vos AP: Extracellular vesicles modulate host-microbe responses by altering TLR2 activity and phagocytosis. PLoS·One. 2014·Feb·20; 9(2):e89121. <·PMID:·24586537·>
• Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hübscher D, Walter L, Streckfuss-Bömeke K, Guan K, Dressel R: Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1.. PLoS·One. 2015·May·7; 10(5):e0125544. <·PMID:·25950680·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN